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$230.00
#225 (5 mg)

Certificate Of Analysis
Form
Storage temp
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... Lipopolysaccharide (LPS) from Salmonella typhimurium was obtained from List Biological Laboratories (Campbell, CA) ...
... Heathy human donor PBMCs ... in 96-well plates were primed with ultra-pure LPS (...; #225, List Biological Laboratories) for 4h at 37°C. PBMCs were then pretreated with control mouse IgG1 or SY2 ...
EAE induction and clinical scoring
EAE/optic neuritis was induced with MOG (MOG33-55, GenScript, cat. no.163913-87-9) peptide and PTX (List Biological Laboratories, Campbell, CA, USA, cat. no. 181) in 9-week-old female mice with a procedure modified from the established protocol.29,30,44 Briefly, 225 μg MOG33-55 peptide emulsified with incomplete Freund's adjuvant (IFA; BD Difco, 263910) and 2.5 mg/mL mycobacterium tuberculosis (BD Difco, 231141) were injected subcutaneously for immunization twice at days 0 and 7, and 500 ng PTX in PBS (2.5 μg/mL) was intraperitoneally injected twice at days 0 and 2 to break down the blood-brain barrier. Mice injected with the same volume of Freund's adjuvant emulsion without MOG33-55 were used as sham controls. ...
Purified LPS and membrane receptor blots
LPS was purified by the hot phenol method previously described41. Purified LPS from E. coli 0111:B4 and Salmonella typhimurium were purchased from List Labs. ...
... LPS from Salmonella Typhimurium was purchased from List Biological Laboratories ...
Materials
LPS from Salmonella typhimurium was purchased from List Biological Laboratories...
Cell culture and stimulation
... For LPS treatments, cells were pretreated with 100 ng/ml LPS for 30 min, followed by addition of FDx. For PMA treatments, cells were pretreated for 15 min with 100 nM PMA, followed by addition of FDx. For IL34 and CXCL12 treatments, cells were treated with FDx and 100 ng/ml IL34 or 50 nM CXCL12. ...
STAR★methods
KEY RESOURCES TABLE
REAGENT OR RESOURCE Source Identifier
... Ultra-pure LPS List Biological Laboratories Cat# 225 ...
ELISA
Mouse peritoneal macrophages were primed with ultra-pure LPS (3 ng/ml; List Biological Lab) for 3 hr and were then stimulated with crystals for 3 hr at 37°C. IL-1β in cell culture supernatants (sup.) was measured by ELISA (R&D Systems).
Cell culture and stimulation
CA-Mφ were generated by priming BMM with 150 U/ml IFN-γ (R&D Systems, Minneapolis, MN) for 6 h, and then stimulating cells overnight with 150 U/ml IFN-γ and 100 ng/ml LPS (from Salmonella typhimurium; no. 225; List Biological Laboratories, Campbell, CA). ...
LPS:
Purified LPS from Salmonella Typhimurium (catalog no. 225) was obtained from List Biological Laboratories, Inc. (Campbell, CA) ...
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List Labs
540 Division Street
Campbell, California
List Biotherapeutics
9800 Crosspoint BIvd Suite 2011
Indianapolis, IN 46256, USA


