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CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature

Louveau, A;Herz, J;Alme, MN;Salvador, AF;Dong, MQ;Viar, KE;Herod, SG;Knopp, J;Setliff, JC;Lupi, AL;Da Mesquita, S;Frost, EL;Gaultier, A;Harris, TH;Cao, R;Hu, S;Lukens, JR;Smirnov, I;Overall, CC;Oliver, G;Kipnis, J;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

... Pertussis toxin (200 ng, List Biologicals) was injected i.p. on day 0 and day 1 after MOG immunization. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 30224810

Loss of HDAC11 ameliorates clinical symptoms in a multiple sclerosis mouse model

Sun, L;Telles, E;Karl, M;Cheng, F;Luetteke, N;Sotomayor, E;Miller, R;Seto, E;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Mice and EAE:

Hdac11 constitutive KO mice were generated at the Institut Clinique de la Souris (ICS) in collaboration with Merck & Co. and obtained via Taconic. These mice were backcrossed with the C57BL/6 background for successive generations and then bred to homozygosity. WT C57BL/6 mice were used as controls for these experiments. To induce EAE, WT and HDAC11 KO female mice between 8 and 12 wk of age were immunized with MOG35–55 peptide emulsified with CFA (Sigma-Aldrich)-containing Mycobacterium tuberculosis (200 ng/ml; Difco). For each animal, 100 μl of emulsion per site was deposited subcutaneously in the upper and lower backs. In addition, each animal was administered pertussis toxin (islet-activating protein, 500 ng, i.p.; List Biological Laboratories) at the time of immunization and again 1 d later. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

Neurocircuitry of fear extinction in adult and juvenile rats

Ganella, DE;Nguyen, LD;Lee-Kardashyan, L;Kim, LE;Paolini, AG;Kim, JH;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae

... 0.2 µl of CTb (10 µg/ml, List Biological Laboratories, CA, USA) was injected into the vHPC, ...

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

PubMed ID: 29898421

Cytomembrane Infused Polymer Accelerating Delivery of Myelin Antigen Peptide to Treat Experimental Autoimmune Encephalomyelitis

Li, J;Qiu, D;Liu, Y;Xiong, J;Wang, Y;Yang, X;Fu, X;Zheng, L;Luo, G;Xing, MMQ;Wu, Y;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE:

... Mice then received 200 ng of pertussis toxin (List Biological Laboratories) intraperitoneally (i.p.) at 4 hours and 24 hours after immunization. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 30265798

Early auto-immune targeting of photoreceptor ribbon synapses in mouse models of multiple sclerosis

Dembla, M;Kesharwani, A;Natarajan, S;Fecher-Trost, C;Fairless, R;Williams, SK;Flockerzi, V;Diem, R;Schwarz, K;Schmitz, F;
Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction of Experimental Auto‐immune Encephalomyelitis (EAE) in female C57BL/6 mice:

Experimental auto‐immune encephalomyelitis (EAE), a frequently used mouse model of multiple sclerosis (Robinson et al, 2014), was induced in female C57BL/6 mice largely as previously described (Williams et al, 2011). For EAE induction, female C57BL/6 mice older than 10 weeks (body weight between 20 and 25 g) were selected. Mice were injected subcutaneously into the axilla and groin with a total of 200 μg of the encephalitogenic MOG35–55 peptide of mouse myelin oligodendrocyte glycoprotein (MEVGWYRSPFSRVVHLYRNGK; > 90% purity, synthesized by Dr. Martin Jung, Institute of Medical Biochemistry and Molecular Biology, Medical School Homburg, Saarland University) in sterile water (2 mg/ml) emulsified with an equal volume of complete Freund adjuvant (CFA), consisting of incomplete Freund adjuvant (iCFA, Sigma; #F5506) to which 10 mg/ml inactivated Mycobacterium tuberculosis were added (Fisher Scientific #10218823), as previously described (Williams et al2011). 200 ng of pertussis toxin (PTX) from Bordetella pertussis (List Biological Laboratories Inc. #181, via Biotrend, Cologne, Germany) in a volume of 100 μl sterile H2O was injected intraperitoneally on the same day (day 0, 1–2 h after MOG35–55 peptide injection) and also on the subsequent day (day 1, 16–20 h after first PTX injection). ...

PubMed ID: 30266776