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Cell type and receptor identity regulate cholera toxin subunit B (CTB) internalization

Sethi, A;Wands, A;Mettlen, M;Krishnamurthy, S;Wu, H;Kohler, J;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

General chemicals:

... CT (azide-free) from V. cholerae used for cAMP experiments was purchased from List Biological Laboratories (Campbell, CA, USA) (catalogue no. 100B).

cAMP measurement:

One hundred microlitres of 50 000 cells ml−1 cell suspensions of individual cell lines were added in wells in white-walled 96-well plates (Thermo-Fisher Scientific, catalogue no. 07-200-628) and cultured for 72 h. The wells were washed twice with PBS and then treated with 0.1 nM CT holotoxin at 37°C for 60 min (List Biologicals) in complete induction buffer provided in the cAMP-Glo kit (Promega, catalogue no. V1501). The assay was performed following the manufacturer's protocol (cAMP-Glo assay; Promega). The luminescence values were obtained using a Synergy Neo microplate reader (BioTek, Winooski, VT, USA).

Activation of constitutive androstane receptor inhibits intestinal CFTR-mediated chloride transport

Kittayaruksakul, S;Sawasvirojwong, S;Noitem, R;Pongkorpsakol, P;Muanprasat, C;Chatsudthipong, V;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Chemicals and reagents:

... Cholera toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). ...

Results - 

Effect of CAR activation on cholera toxin (CT)-induced Cl secretion in T84 cells:

As activation of CFTR-dependent Cl secretion is involved in the pathogenesis of secretory diarrhea especially cholera [23], the therapeutic potential of CAR agonist on cholera toxin (CT)-induced Cl secretion by T84 cell monolayers was investigated using short-circuit current analysis. Snapwell inserts containing confluent T84 cells were treated with 1 μM CITCO for 24 h followed by mounting in an Ussing chamber. Then, 1 μg/ml of CT was added into apical Kreb’s buffer to stimulate Clsecretion. As demonstrated in Fig. 8, CT-induced Cl secretion in T84 cells was significantly reduced by CITCO (78.6 ± 6.0% of control).

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

Akt-1 and Akt-2 Differentially Regulate the Development of Experimental Autoimmune Encephalomyelitis by Controlling Proliferation of Thymus-Derived Regulatory T Cells

Ouyang, S;Zeng, Q;Tang, N;Guo, H;Tang, R;Yin, W;Wang, A;Tang, H;Zhou, J;Xie, H;Langdon, WY;Yang, H;Zhang, J;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

... Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 30692211

Aromatic substances in wheat malt inducing antisecretory factor and resistance to diarrhoea

Johansson, E;Lange, S;Lönnroth, I;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Chemicals and cereals:

... Cholera toxin was obtained from List Biological Laboratories ...

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

Spatial Heterogeneity of Lung Strain and Aeration and Regional Inflammation During Early Lung Injury Assessed with PET/CT

Motta-Ribeiro, G;Winkler, T;Hashimoto, S;Vidal Melo, MF;
Product: LPS from Escherichia coli O55:B5

... After baseline measurements, endotoxin was intravenously infused throughout the experiment to model either moderate (10 ng/kg/min, Escherichia coli O55:B5, List Biologic Laboratories Inc, Campbell, California) or mild (2.5 ng/kg/min) endotoxemic states. ...

PubMed ID: 30057194