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Anthrax Protective Antigen, Activated (PA 63) from B. anthracis
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Products are for research purposes only and are
not for use in humans or as diagnostic agents.
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$630.00
#174 (0.5 mg)
Purity
>95%
Form
lyophilized powder
Storage temp
2-8°C
Chloroquine-analogues block anthrax protective antigen channels in steady-state and kinetic studies
Beitzinger, Kronhardt, Benz
Toxicology (2023) 492, 153547 DOI: 10.1016/j.tox.2023.153547
Beitzinger, Kronhardt, Benz
Toxicology (2023) 492, 153547 DOI: 10.1016/j.tox.2023.153547
Snippet:
... Recombinant, nicked PA63 from B. anthracis was obtained from List Biological Laboratories Inc., Campbell, CA. ...
Novel role of GPR109A in thymic regulatory T cell development
Macia, Ni, Tan et al
Research Square (2023)
Macia, Ni, Tan et al
Research Square (2023)
Snippet:
... Upon immunization and 2-day post immunization, mice were also intravenously injected with 300ng/mouse pertussis toxin (List Biological Laboratories) ...
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
Solution Structures of Bacillus anthracis Protective Antigen Proteins Using Small Angle Neutron Scattering and Protective Antigen 63 Ion Channel Formation Kinetics
Michelman-Ribeiro, Rubinson, Silin et al
Toxins (Basel) (2021) 13 (12) DOI: 10.3390/toxins13120888
Michelman-Ribeiro, Rubinson, Silin et al
Toxins (Basel) (2021) 13 (12) DOI: 10.3390/toxins13120888
Cited by 4
Snippet:
... Recombinant B. anthracis protective antigen 63 (PA63, List Biological Laboratories, Campbell, CA, USA)...
Multiplexed, High-Sensitivity Measurements of Antibody Affinity Using Interferometric Reflectance Imaging Sensor
Marn, Needham, Chiodi et al
Biosensors (Basel) (2021) 11 (12) DOI: 10.3390/bios11120483
Marn, Needham, Chiodi et al
Biosensors (Basel) (2021) 11 (12) DOI: 10.3390/bios11120483
Cited by 12
Snippet:
2.3. Reagents
... Anthrax protective antigen, PA63, was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). ...
Anthrax Edema and Lethal Toxins Differentially Target Human Lung and Blood Phagocytes
Patel, Booth, Dozmorov et al
Toxins (Basel) (2020) 12 (7) DOI: 10.3390/toxins12070464
Patel, Booth, Dozmorov et al
Toxins (Basel) (2020) 12 (7) DOI: 10.3390/toxins12070464
Cited by 13
Snippet:
Protective Antigen Binding Assays
For all PA binding assays, activated PA (63 kDa, List Biological Laboratories, Inc.; Campbell, CA, USA) was labeled using an Alexa Fluor® 647 Microscale Protein Labeling Kit (Molecular Probes; Waltham, MA, USA) following the manufacturer's instructions. RAW 264.7, human BAL cells, and human leukocytes were processed as described for anthrax surface receptor expression through the point of Fc receptor blocking. At this point, cells were either left untreated, or incubated with 3000 or 10,000 ng/mL unlabeled PA as indicated for 30 min on ice. Cells were then exposed to AF647-labeled PA at 10, 100, or 1000 ng/mL for an additional 30 min on ice. In the case of lung and blood cells, antibodies used for cell subset-specific identification were also added at the same time as AF647-PA. All samples were centrifuged at 300× g for 5 min and washed twice with 1X PBS-2. Samples were fixed with 2% paraformaldehyde prior to flow cytometry.
Exploring the Nature of Cationic Blocker Recognition by the Anthrax Toxin Channel
Momben Abolfath, Kolberg, Karginov et al
Biophys. J. (2019) 117 (9), 1751-1763 DOI: 10.1016/j.bpj.2019.08.041
Momben Abolfath, Kolberg, Karginov et al
Biophys. J. (2019) 117 (9), 1751-1763 DOI: 10.1016/j.bpj.2019.08.041
Snippet:
Chemicals
... PA63 protein was prepared from PA83 using furin (53) or purchased from List Biological Laboratories (Campbell, CA) in the purified form. ...
Zeptomole per milliliter detection and quantification of edema factor in plasma by LC-MS/MS yields insights into toxemia and the progression of inhalation anthrax
Lins, Boyer, Kuklenyik et al
Anal Bioanal Chem (2019)
Lins, Boyer, Kuklenyik et al
Anal Bioanal Chem (2019)
Snippet:
... Recombinant EF was produced as previously described. Activated recombinant PA (PA63) was from List Biological Laboratories (Campbell, CA). ...
Effect of late endosomal DOBMP lipid and traditional model lipids of electrophysiology on the anthrax toxin channel activity
Kalu, Atsmon-Raz, Abolfath et al
Biochimica et Biophysica Acta (BBA) - Biomembranes (2018)
Kalu, Atsmon-Raz, Abolfath et al
Biochimica et Biophysica Acta (BBA) - Biomembranes (2018)
Snippet:
... PA 63 was prepared from PA 83 using furin [52] or purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) in the purified form ...
Effect of endosomal acidification on small ion transport through the anthrax toxin PA63 channel
Kalu, Alcaraz, Yamini et al
FEBS Lett. (2017)
Kalu, Alcaraz, Yamini et al
FEBS Lett. (2017)
Snippet:
... Given the important role of pH change in the anthrax toxin internalization process, the current study is designed to address the disparity.
Material and Methods:
PA63 was purchased from List Biological Laboratories, Inc. (Campbell, CA). ...
Laboratory evolution of artificially expanded DNA gives redesignable aptamers that target the toxic form of anthrax protective antigen
Biondi, Lane, Das et al
Nucleic Acids Res. (2016) 44 (20), 9565-9577 DOI: 10.1093/nar/gkw890
Biondi, Lane, Das et al
Nucleic Acids Res. (2016) 44 (20), 9565-9577 DOI: 10.1093/nar/gkw890
Snippet:
Materials:
... PA from B. anthracis in its holo form (PA83) and cleaved form (PA63) where purchased from List Biological Laboratories, ...
In vitro selection scheme:
The AEGIS-LIVE experiment was performed on a synthetic library of GACTZP oligonucleotides containing 25 randomized positions flanked by two primer binding sites (59 nt in length, 5-AGAGAGCGTCGTGTGGA-N25-TGAGGAGGTGCGCAAGT-3).
PA was presented immobilized on magnetic beads...
Filter binding assays:
5-32P-labeled aptamer (1 l each) was denatured/renatured in 1 PBS (30 l), and incubated (45 min, RT) with increasing concentrations of PA63 or PA83, from 0 to 12 M. To capture the PA and PA-aptamer complex, aliquots (5 l) from each binding reaction were spotted on a nitrocellulose membrane on top of a charged nylon membrane (Whatman) in a dot-blot minifold vacuum device (Millipore) (27). Membranes were washed ...
Determination of the affinity constant with bead-based assay:
After being coupled to PA63 (1.6 nmoles), the beads (200 l, as during selection) were divided into aliquots (20 l each) and each fraction was incubated with increasing concentrations of ...
Enzymatic footprinting:
For DNase I digestion of PA1 and PA1T4 in the presence of PA63 or PA83, the aptamer was denatured (85C, 5 min) followed by slow (0.1/s) cooling to 24C to allow refolding. 532P-PA1 with 05 M PA63 or PA83 was incubated with tumbling for 45 min, and then treated with DNase I nuclease (15 min at RT). The digested products were resolved on 18% denaturing PAGE. ...
Electrophysiology assays:
LF N-terminal domain (LFN) and PA were expressed and purified as described (21). To form heptameric PA oligomers, PA was nicked with trypsin (1:1000 w/w) at room temperature in buffer (20 mM TrisCl, pH 8) ...
Showing 1-10 of 24 papers.
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