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Sensory Neurons of the Dorsal Root Ganglia Become Hyperexcitable in a T-Cell-Mediated MOG-EAE Model of Multiple Sclerosis

Yousuf, MS;Noh, MC;Friedman, TN;Zubkow, K;Johnson, JC;Tenorio, G;Kurata, HT;Smith, PA;Kerr, BJ;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Multiple sclerosis (MS) is an autoimmune, demyelinating disease of the central nervous system. Patients with MS typically present with visual, motor, and sensory deficits. However, an additional complication of MS in large subset of patients is neuropathic pain. To study the underlying immune-mediated pathophysiology of pain in MS we employed the myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalitis (EAE) model in mice. Since sensory neurons are crucial for nociceptive transduction, we investigated the effect of this disease on sensory neurons of the lumbar dorsal root ganglia (DRG). Here, we report the disease was associated with activation of the complement system and the NLRP3 inflammasome in the DRG. We further observe a transient increase in the number of complement component 5a receptor 1-positive (C5aR1+) immune cells, CD4+ T-cells, and Iba1+ macrophages in the DRG. The absence of any significant change in the levels of mRNA for myelin proteins in the DRG and the sciatic nerve suggests that demyelination in the PNS is not a trigger for the immune response in the DRG. However, we did observe an induction of activating transcription factor 3 (ATF3) at disease onset and chronic disruption of cytoskeletal proteins in the DRG demonstrating neuronal injury in the PNS in response to the disease. Electrophysiological analysis revealed the emergence of hyperexcitability in medium-to-large (≥26 µm) diameter neurons, especially at the onset of MOG-EAE signs. These results provide conclusive evidence of immune activation, neuronal injury, and peripheral sensitization in MOG-EAE, a model classically considered to be centrally mediated.

PubMed ID: 30957012
353135312019-04-172019-04-1710:30:1510:30:152019-04-192019-04-1914:01:0214:01:02Yousuf, MS;Noh, MC;Friedman, TN;Zubkow, K;Johnson, JC;Tenorio, G;Kurata, HT;Smith, PA;Kerr, BJ;Yousuf, MS;Noh, MC;Friedman, TN;Zubkow, K;Johnson, JC;Tenorio, G;Kurata, HT;Smith, PA;Kerr, BJ;20192019Sensory Neurons of the Dorsal Root Ganglia Become Hyperexcitable in a T-Cell-Mediated MOG-EAE Model of Multiple SclerosisSensory Neurons of the Dorsal Root Ganglia Become Hyperexcitable in a T-Cell-Mediated MOG-EAE Model of Multiple SclerosiseNeuroeNeuro66223095701230957012

Multiple sclerosis (MS) is an autoimmune, demyelinating disease of the central nervous system. Patients with MS typically present with visual, motor, and sensory deficits. However, an additional complication of MS in large subset of patients is neuropathic pain. To study the underlying immune-mediated pathophysiology of pain in MS we employed the myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalitis (EAE) model in mice. Since sensory neurons are crucial for nociceptive transduction, we investigated the effect of this disease on sensory neurons of the lumbar dorsal root ganglia (DRG). Here, we report the disease was associated with activation of the complement system and the NLRP3 inflammasome in the DRG. We further observe a transient increase in the number of complement component 5a receptor 1-positive (C5aR1+) immune cells, CD4+ T-cells, and Iba1+ macrophages in the DRG. The absence of any significant change in the levels of mRNA for myelin proteins in the DRG and the sciatic nerve suggests that demyelination in the PNS is not a trigger for the immune response in the DRG. However, we did observe an induction of activating transcription factor 3 (ATF3) at disease onset and chronic disruption of cytoskeletal proteins in the DRG demonstrating neuronal injury in the PNS in response to the disease. Electrophysiological analysis revealed the emergence of hyperexcitability in medium-to-large (≥26 µm) diameter neurons, especially at the onset of MOG-EAE signs. These results provide conclusive evidence of immune activation, neuronal injury, and peripheral sensitization in MOG-EAE, a model classically considered to be centrally mediated.

Multiple sclerosis (MS) is an autoimmune, demyelinating disease of the central nervous system. Patients with MS typically present with visual, motor, and sensory deficits. However, an additional complication of MS in large subset of patients is neuropathic pain. To study the underlying immune-mediated pathophysiology of pain in MS we employed the myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalitis (EAE) model in mice. Since sensory neurons are crucial for nociceptive transduction, we investigated the effect of this disease on sensory neurons of the lumbar dorsal root ganglia (DRG). Here, we report the disease was associated with activation of the complement system and the NLRP3 inflammasome in the DRG. We further observe a transient increase in the number of complement component 5a receptor 1-positive (C5aR1+) immune cells, CD4+ T-cells, and Iba1+ macrophages in the DRG. The absence of any significant change in the levels of mRNA for myelin proteins in the DRG and the sciatic nerve suggests that demyelination in the PNS is not a trigger for the immune response in the DRG. However, we did observe an induction of activating transcription factor 3 (ATF3) at disease onset and chronic disruption of cytoskeletal proteins in the DRG demonstrating neuronal injury in the PNS in response to the disease. Electrophysiological analysis revealed the emergence of hyperexcitability in medium-to-large (≥26 µm) diameter neurons, especially at the onset of MOG-EAE signs. These results provide conclusive evidence of immune activation, neuronal injury, and peripheral sensitization in MOG-EAE, a model classically considered to be centrally mediated.

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EAE:

... A set of CFA-only administered mice were used as control for EAE induction. Two intraperitoneal injections of pertussis toxin, Bordatella pertussis, (List Biological Labs) were also administered to all mice on the day of induction and 48 hours thereafter. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

EAE:

... A set of CFA-only administered mice were used as control for EAE induction. Two intraperitoneal injections of pertussis toxin, Bordatella pertussis, (List Biological Labs) were also administered to all mice on the day of induction and 48 hours thereafter. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

http://www.eneuro.org/content/early/2019/03/20/ENEURO.0024-19.2019.abstracthttp://www.eneuro.org/content/early/2019/03/20/ENEURO.0024-19.2019.abstract2019-04-012019-04-0110.1523/ENEURO.0024-19.201910.1523/ENEURO.0024-19.2019Pertussis Toxin from B. pertussis, Lyophilized in BufferPertussis Toxin from B. pertussis, Lyophilized in Bufferbradley.kerr@ualberta.cabradley.kerr@ualberta.caActivation;Analysis;Autoimmune;Biological;Cell;Central Nervous System;Complement;Component;Control;Disease;EAE;Experimental;Factor;Immune Cells;Immune response;Induction;List;List Biological;mRNA;MS;Nerve;Pertussis;Positive;Receptor;Response;Sensory;Study;T-cell;Toxin;Transcription;Transduction;eNeuroActivation;Analysis;Autoimmune;Biological;Cell;Central Nervous System;Complement;Component;Control;Disease;EAE;Experimental;Factor;Immune Cells;Immune response;Induction;List;List Biological;mRNA;MS;Nerve;Pertussis;Positive;Receptor;Response;Sensory;Study;T-cell;Toxin;Transcription;Transduction;eNeuro180180sensory-neurons-of-the-dorsalsensory-neurons-of-the-dorsal