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Bystander-mediated stimulation of proteolipid protein-specific regulatory T (Treg) cells confers protection against experimental autoimmune encephalomyelitis (EAE) via TGF-β

Jun, S;Ochoa-Repáraz, J;Zlotkowska, D;Hoyt, T;Pascual, DW;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

To assess the potency of regulatory T (Treg) cells induced against an irrelevant Ag, mice were orally vaccinated with Salmonella expressing Escherichia coli colonization factor antigen I fimbriae. Isolated CD25+ and CD25−CD4+ T cells were adoptively transferred to naive mice, and Treg cells effectively protected against experimental autoimmune encephalomyelitis (EAE), unlike Treg cells from Salmonella vector-immunized mice. This protection was abrogated upon in vivo neutralization of TGF-β, resulting in elevated IL-17 and loss of IL-4 and IL-10 production. Thus, Treg cells induced to irrelevant Ags offer a novel approach to treat autoimmune diseases independent of auto-Ag.

262526252017-06-052017-06-0511:03:0511:03:052018-04-252018-04-2510:45:5810:45:58Jun, S;Ochoa-Repáraz, J;Zlotkowska, D;Hoyt, T;Pascual, DW;Jun, S;Ochoa-Repáraz, J;Zlotkowska, D;Hoyt, T;Pascual, DW;20122012Bystander-mediated stimulation of proteolipid protein-specific regulatory T (Treg) cells confers protection against experimental autoimmune encephalomyelitis (EAE) via TGF-βBystander-mediated stimulation of proteolipid protein-specific regulatory T (Treg) cells confers protection against experimental autoimmune encephalomyelitis (EAE) via TGF-βJournal Of NeuroimmunologyJournal Of Neuroimmunology39–4739–472452452-Jan2-Jan

To assess the potency of regulatory T (Treg) cells induced against an irrelevant Ag, mice were orally vaccinated with Salmonella expressing Escherichia coli colonization factor antigen I fimbriae. Isolated CD25+ and CD25−CD4+ T cells were adoptively transferred to naive mice, and Treg cells effectively protected against experimental autoimmune encephalomyelitis (EAE), unlike Treg cells from Salmonella vector-immunized mice. This protection was abrogated upon in vivo neutralization of TGF-β, resulting in elevated IL-17 and loss of IL-4 and IL-10 production. Thus, Treg cells induced to irrelevant Ags offer a novel approach to treat autoimmune diseases independent of auto-Ag.

To assess the potency of regulatory T (Treg) cells induced against an irrelevant Ag, mice were orally vaccinated with Salmonella expressing Escherichia coli colonization factor antigen I fimbriae. Isolated CD25+ and CD25−CD4+ T cells were adoptively transferred to naive mice, and Treg cells effectively protected against experimental autoimmune encephalomyelitis (EAE), unlike Treg cells from Salmonella vector-immunized mice. This protection was abrogated upon in vivo neutralization of TGF-β, resulting in elevated IL-17 and loss of IL-4 and IL-10 production. Thus, Treg cells induced to irrelevant Ags offer a novel approach to treat autoimmune diseases independent of auto-Ag.

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Adoptive transfer, EAE challenge, and TGF-β neutralization:

To isolate Treg cells, CD4+CD25 and CD4+CD25+ T cells were obtained from spleens and head and neck lymph nodes (HNLNs) from H696- or H647-vaccinated mice. ...

To induce EAE, SJL females were given s.c 200 μg of the encephalitogenic PLP peptide (PLP139–151; HSLGKWLGHPDKF, HPLC-purified to >90%; Biosynthesis, Inc., Lewisville, TX) in 200 μl of complete Freund’s adjuvant. On days 0 and 2, mice received i.p. 200 ng of Bordetella pertussis toxin (List Biological Laboratories; Campbell, CA), as previously described ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

Adoptive transfer, EAE challenge, and TGF-β neutralization:

To isolate Treg cells, CD4+CD25 and CD4+CD25+ T cells were obtained from spleens and head and neck lymph nodes (HNLNs) from H696- or H647-vaccinated mice. ...

To induce EAE, SJL females were given s.c 200 μg of the encephalitogenic PLP peptide (PLP139–151; HSLGKWLGHPDKF, HPLC-purified to >90%; Biosynthesis, Inc., Lewisville, TX) in 200 μl of complete Freund’s adjuvant. On days 0 and 2, mice received i.p. 200 ng of Bordetella pertussis toxin (List Biological Laboratories; Campbell, CA), as previously described ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3328618/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3328618/2012-03-122012-03-12https://doi.org/10.1016/j.jneuroim.2012.02.003https://doi.org/10.1016/j.jneuroim.2012.02.003Pertussis Toxin from B. pertussis, Lyophilized in BufferPertussis Toxin from B. pertussis, Lyophilized in Bufferdpascual@montana.edudpascual@montana.eduAdjuvant;Antigen;Assess;Autoimmune;Biological;Bordetella pertussis;Colonization;EAE;Encephalomyelitis;Escherichia coli;Experimental;Experimental Autoimmune Encephalomyelitis;Factor;Fimbriae;IL;Isolated;List;List Biological;Neutralization;Neutralization;Novel;Pertussis;Potency;Production;Protein;Regulatory;Specific;Toxin;Treat;Vector;Journal Of NeuroimmunologyAdjuvant;Antigen;Assess;Autoimmune;Biological;Bordetella pertussis;Colonization;EAE;Encephalomyelitis;Escherichia coli;Experimental;Experimental Autoimmune Encephalomyelitis;Factor;Fimbriae;IL;Isolated;List;List Biological;Neutralization;Neutralization;Novel;Pertussis;Potency;Production;Protein;Regulatory;Specific;Toxin;Treat;Vector;Journal Of Neuroimmunology180180bystander-mediated-stimulation-of-proteolipid-protein-specificbystander-mediated-stimulation-of-proteolipid-protein-specific