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Page 652 out of 659

Detection, prevention, and treatment systems for anthrax

Morrow P, Morrow J, Kang A, Wang F, Jiang I, Sawada R, Scholz W, Menos 
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

... Flat bottom microtiter plates (Nunc F96 Maxisorp) were coated with 50 μl of Bacillus anthracis Protective Antigen (PA) or Lethal Factor (LF)(List Biological Laboratories (Campbell, Calif.) at a concentration of 1 μg/mL in PBS overnight at 4° C. ...

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Sensitive and Specific Assay to Measure Endoprotease Activity of Botulinum Toxin Type A Holotoxin in Milk.

Shine, N.; Eaton, L.; Crawford, K.
Product: Botulinum Neurotoxin Type A from Clostridium botulinum

Materials:

Botulinum Neurotoxin Type A (Prod #130A) and the recombinant light chain of the Type A neurotoxin (Prod #610A) are products of List Biological Laboratories.

Methods - The above materials were used in the following (refer to poster for details):

  • HPLC
  • Digestion

Correction of Murine Hemophilia a by Hematopoietic Stem Cell Gene Therapy

Moayeri M, Hawley TS, Hawley RG
Product: Tetanus Toxoid from Clostridium tetani

FVIII and Tetanus Toxoid Immunizations:

... Two of the 800 cGy-irradiated mice (mice #26 and #27) and four naive E16 mice were immunized with tetanus toxoid (List Biological Laboratories, Inc., Campbell, CA, USA) 3 weeks after the final FVIII injection as follows: 4 μg tetanus toxoid was mixed with 1 mg Al (OH)3adjuvant (Alhydrogel 2%; Accurate Chemical & Scientific Corporation, Westbury, NY, USA), diluted in sterile saline to a total volume of 108 μl and injected intraperitoneally. Two weeks later, sera from these mice was assessed for the presence of anti-tetanus toxoid antibodies by ELISA (described below).

PubMed ID: 16226058

Anthrax Lethal Toxin-Mediated Killing of Human and Murine Dendritic Cells Impairs the Adaptive Immune Response

Alileche A, Serfass ER, Muehlbauer SM, Porcelli SA, Brojatsch J
Product: Anthrax Lethal Factor (LF),Recombinant from B. anthracis

Materials and Methods:

... Recombinant anthrax LF was obtained from List Biological Laboratories (Campbell, California, United States). PA was generously provided by Dr. Steven Leppla (National Institutes of Health). PA and LF were reconstituted in water at 500 ng/ml and 250 ng/ml, respectively [35]. Recombinant LF and PA were produced in B. anthracis and were free of LPS contamination as indicated by the manufacturer, and by a lack of CD86 up-regulation on immature cells, as measured by flow cytometry.

Cell death and viability assays:

Cell viability was measured by analysis of MTT cleavage to formazan by succinate dehydrogenases in living cells [37]. For the colorimetric MTT assay, cells were exposed to LT (500 ng/ml PA and 250 ng/ml LF), and the MTT solution (5 mg/ml MTT in PBS) was added directly to wells and incubated at 37 °C for 4 h. The dye was solubilized with acidic isopropanol (25 mM HCl and 0.5% SDS in isopropanol), and the absorbance was measured at 570 nm. ...

Western blotting:

Cells were cultured in 24-well plates and treated with LT.  ...

In vivo assay:

Ten-week-old female BALB/c mice were injected intraperitoneally with LT [3,4]. Mice were sacrificed 0, 3, 6, and 24 h after LT injection. Spleens were harvested and treated with 400 U/ml collagenase D (Roche) to release DCs. Levels of splenic DCs (CD11c+, MHC class II+), macrophages (CD11b+, MHC class II+), and circulating B cells (B220+) and T cells (CD3+) were determined by flow cytometry using fluorescently labeled antibodies to surface markers.

 

LT (Lethal Toxin) = Lethal Factor (LF) + Protective Antigen (PA)

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

PubMed ID: 16254597

Characteristics of Physical Characteristics of rLF and rPA: Effects on Enzymatic : Effects on Enzymatic Activity and Binding

Shine, N., Le, C., Eaton, L., Crawford, K.
Product: Unquenched Calibration Peptide for MAPKKide® 530 for Anthrax Lethal Factor

Materials:

Recombinant lethal factor (Prod #172), protective antigen (Prod #171), MAPKKideTM (Prod #530), and MAPKKideTM Unquenched Calibration Peptide (Prod #539) are products of List Biological Laboratories.

Methods - The above materials were used in the following (refer to poster for details):

  • HPLC
  • Enzymatic activity
  • Continuous fluorimetric assays
  • LF-PA63 binding assay
  • LF enzymatic activity as a function of ZnCl2