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Page 652 out of 685

pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen

Matsumoto, Y;Zhang, Q;Akita, K;Nakada, H;Hamamura, K;Tokuda, N;Tsuchida, A;Matsubara, T;Hori, T;Okajima, T;Furukawa, K;Urano, T;Furukawa, K;
Product: Cholera Toxin B Subunit Biotin Conjugate

... Cholera toxin B (CTB) subunit-biotin conjugates was from List Biological Laboratories, Inc. (Campbell, CA). ...

PubMed ID: 22306014

Proteomic identification of immunoproteasome accumulation in formalin-fixed rodent spinal cords with experimental autoimmune encephalomyelitis

Jain, MR;Li, Q;Liu, T;Rinaggio, J;Ketkar, A;Tournier, V;Madura, K;Elkabes, S;Li, H;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE induction, disability evaluation and tissue harvesting:

... For the mouse model, 8–10 weeks-old female C57BL/6 mice (Taconic, Hudson, NY) were immunized subcutaneously in the flank with 200 µg myelin oligodendrocyte glycoprotein peptide 35–55 (MEVGWYRSPFSRVVHLYRNGK; Stanford PAN Facility, Stanford, CA) emulsified in CFA that had been supplemented with M. tuberculosis strain H37RA (MT; Difco Laboratories, Detroit, MI) at 7 mg/ml. All mice also received an intraperitoneal injection of 350 ng pertussis toxin (List Biological, Campbell, CA) at 0 and 48 h post-immunization. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22188123

A transgenic model of central nervous system autoimmunity mediated by CD4+ and CD8+ T and B cells.

Anderson, AC;Chandwaskar, R;Lee, DH;Sullivan, JM;Solomon, A;Rodriguez-Manzanet, R;Greve, B;Sobel, RA;Kuchroo, VK;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Degranulation assay:

Mice were immunized with 10 mg MOG 35–55 peptide emulsified in CFA (Difco) supplemented with 4 mg/ml Mycobacterium tuberculosis (H37RA; Difco). Each mouse also received 200 ng pertussis toxin (List Biological Laboratories) intravenously on days 0 and 2. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22279107

Afferent and efferent projections of the mesopallium in the pigeon (Columba livia)

Atoji, Y;Wild, JM;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

... injections of two different tracers into subdivisions of the mesopallium, ... and cholera toxin B subunit (CTB, choleragenoid; List Biological Laboratories, Campbell, CA ...

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 μl of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA, 0.003 M NaN3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

PubMed ID: 21935938

Bacillus anthracis lethal toxin alters regulation of visceral sympathetic nerve discharge

Garcia, AA;Fels, RJ;Mosher, LJ;Kenney, MJ;
Product: Anthrax Lethal Factor (LF),Recombinant from B. anthracis

Experimental protocols:

After completion of the surgical procedures, anesthetized rats were allowed to stabilize for 60 min before initiation of experimental protocols. As described by Cui et al. (4, 6), rats received LeTx [LF (100 μg/kg) + PA (200 μg/kg) infused at 0.5 ml/h] or vehicle (PA + PBS with 1% BSA or PBS with 1% BSA infused at 0.5 ml/h). LF and PA are recombinant proteins prepared from B. anthracis and purchased from List Biological Laboratories. Experiments were completed in intact and SAD rats. LeTx or vehicle was infused intravenously via a femoral venous catheter, and the dose of LeTx was chosen on the basis of the time course of physiological responses to intravenous LeTx infusions as reported by Cui and colleagues (6). MAP, HR, and SND were recorded continuously during intravenous LeTx or vehicle infusions. The maximum infusion duration was 6 h; however, because of LeTx-induced reductions in arterial blood pressure, the majority of experiments were terminated before 6 h. Additional experiments involved the intravenous infusion of sodium nitroprusside (SNP) at a dose (20 μg/kg) that reduced basal levels of MAP to 50–60 mmHg. At the end of experiments, rats were euthanized by an overdose of methohexital sodium (150 mg/kg iv).

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

PubMed ID: 22114180