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Page 644 out of 659

Antibodies recognizing protective pertussis toxin epitopes are preferentially elicited by natural infection versus acellular immunization

Sutherland JN, Chang C, Yoder SM, Rock MT, Maynard JA.
Product: Pertussis Toxin from B. pertussis (in Glycerol)

Protein expression and purification:

PTx (holotoxin) in glycerol was purchased from List Biological Laboratories, Inc. (Campbell, CA). ...

PTx toxoiding and characterization:

To detoxify PTx, the protocol developed by Fowler et al., similar to that used in current acellular vaccine formulations, was employed (11). Lyophilized, salt-free PTx (List Labs) was dissolved in phosphate-buffered saline (PBS) with 0.5% (wt/vol) formaldehyde and 0.05 M lysine for a final PTx concentration of 0.5 μM. ...

PubMed ID: 21508166

Defining the Strain-Dependent Impact of the Staphylococcal Accessory Regulator (sarA) on the Alpha-Toxin Phenotype of Staphylococcus aureus

Zielinska AK, Beenken KE, Joo HS, Mrak LN, Griffin LM, Luong TT, Lee CY, Otto M, Shaw LN, Smeltzer MS
Product: Alpha Toxin from Staphylococcus aureus

Western blotting:

The production of alpha-toxin was assessed by Western blotting of standardized cell-free supernatants using rabbit polyclonal anti-alpha-toxin IgG antibody (Sigma Chemical Co., St. Louis, MO) as previously described (48). For quantitative comparisons, 50 ng of purified alpha-toxin (List Biological Laboratories, Campbell, CA) was included as an internal standard on each gel. ...

Product #120, Alpha Toxin from Staphylococcus aureus, has been discontinued.  Large quantities may be obtained through Bulk Requests and Custom Orders.

PubMed ID: 21478342

HPLC Method to Assay the ADP - Ribosyltransferase Activity of Cholera Toxin

Suryadi, K.; Shine, N.
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

HPLC Method to Assay the ADP - Ribosyltransferase Activity of Cholera Toxin

Materials: Cholera Toxin (Prod # 100B) and CTB (Prod # 104) are products of List Biological Laboratories, Inc.

Low level bacterial endotoxin activates two distinct signaling pathways in human peripheral blood mononuclear cells

Blomkalns AL, Stoll LL, Shaheen W, Romig-Martin SA, Dickson EW, Weintraub NL, Denning GM
Product: LPS from Escherichia coli K12 strain LCD25


The endotoxin (LPS) from E. coli K12 LCD25 was purchased from List Biological Laboratories. All LPS preparations from List Biological Laboratories, Inc., are essentially free of nucleic acid and protein and are chemically characterized with respect to their phosphate and 2-keto-3-deoxyoctonate (KDO) contents. For these studies, the purchased LPS preparation was further purified by a phenol re-extraction method to eliminate residual protein contamination prior to use in our experimental models. ...

PubMed ID: 21352551

A non-glycosylated, plant-produced human monoclonal antibody against anthrax protective antigen protects mice and non-human primates from B. anthracis spore challenge

Mett V, Chichester JA, Stewart ML, Musiychuk K, Bi H, Reifsnyder CJ, Hull AK, Albrecht MT, Goldman S, Baillie LW, Yusibov V
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

In vitro characterization of plant-produced mAbs:

ELISA was performed as previously described in reference 36, with slight modifications. Plates were coated with 1 μg/mL of recombinant B. anthracis PA (List Biological Laboratories Inc., Campbell, CA). ...

PubMed ID: 21270531