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Tetanus light chain is manufactured as a recombinant protein. Each lot of tetanus light chain is tested for enzymatic activity in an endopeptidase reaction with the recombinant eukaryotic substrate GST synaptobrevin 2. Tetanus light chain is a non-toxic protein that retains the enzymatic activity encoded by the holotoxin. With this product, there is no chance of contamination by heavy chain since only the light chain is encoded and expressed. Light chain is unable to gain access to intracellular targets without microinjection.