Freeze drying (or lyophilization) of bacteria interrupts the metabolism of a bacterial culture putting in a dry powder resting state. Bacteria are brought back from the dry powder with the addition of water and nutrient media. After lyophilization, In the dry state bacteria may be stored for a long period and handled at room temperature.
Difficulties in the freeze drying process lie in removing water while retaining the ability of the bacteria to come to life when rehydrated. Various sugars, proteins, small molecules and salts are used to formulate and protect the bacteria during the drying process and subsequent rehydration. Equally important are the methods used for freezing and evaporating the water. A well designed formulation and lyophilization cycle can produce bacterial product which can be stored and revived on cue.
Bacterial stocks, proteins and LPS have been formulated and lyophilized as part of our standard product line at List Labs for more than thirty years. Let us put our experience to work in formulating and drying your cell bank or live biotherapeutic.