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Bordetella pertussis antigens encapsulated into N-trimethyl chitosan nanoparticulate systems as a novel intranasal pertussis vaccine

Najminejad, H;Kalantar, SM;Mokarram, AR;Dabaghian, M;Abdollahpour-Alitappeh, M;Ebrahimi, SM;Tebianian, M;Fasihi Ramandi, M;Sheikhha, MH;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

The mucosal immune system serves as the first line of defense against Bordetella pertussis. Intranasal vaccination, due to its potential to induce systemic and mucosal immune responses, appears to prevent the initial adherence and colonization of the bacteria at the first point of contact. In the present study, two B. pertussis antigens, pertussis Toxoid (PTd) and Filamentous hemagglutinin (FHA), which play a very significant role in virulence and protection against pertussis, were encapsulate into N-trimethyl chitosan (TMC) nanoparticulate systems. After preparation of TMC nanoparticles (NPs), the NPs were characterized and their ability to induce efficient immune responses against B. pertussis was studied in a mouse model. Our findings showed that PTd + FHA-loaded TMC NPs have strong ability to induce IL-4, IL-17, IFN-γ, IgG, and IgA in the mouse model. Results from this study suggest that nasal administration of the PTd + FHA-loaded TMC NPs induced not only a systemic immune response but also a local mucosal response, which may improve the efficacy of pertussis prevention through respiratory tract transmission.

PubMed ID: 31240957
361236122019-07-152019-07-1516:21:5216:21:522019-07-252019-07-2512:23:0312:23:03Najminejad, H;Kalantar, SM;Mokarram, AR;Dabaghian, M;Abdollahpour-Alitappeh, M;Ebrahimi, SM;Tebianian, M;Fasihi Ramandi, M;Sheikhha, MH;Najminejad, H;Kalantar, SM;Mokarram, AR;Dabaghian, M;Abdollahpour-Alitappeh, M;Ebrahimi, SM;Tebianian, M;Fasihi Ramandi, M;Sheikhha, MH;20192019Bordetella pertussis antigens encapsulated into N-trimethyl chitosan nanoparticulate systems as a novel intranasal pertussis vaccineBordetella pertussis antigens encapsulated into N-trimethyl chitosan nanoparticulate systems as a novel intranasal pertussis vaccineArtificial Cells, Nanomedicine, And BiotechnologyArtificial Cells, Nanomedicine, And Biotechnology2605-26112605-26114747113124095731240957

The mucosal immune system serves as the first line of defense against Bordetella pertussis. Intranasal vaccination, due to its potential to induce systemic and mucosal immune responses, appears to prevent the initial adherence and colonization of the bacteria at the first point of contact. In the present study, two B. pertussis antigens, pertussis Toxoid (PTd) and Filamentous hemagglutinin (FHA), which play a very significant role in virulence and protection against pertussis, were encapsulate into N-trimethyl chitosan (TMC) nanoparticulate systems. After preparation of TMC nanoparticles (NPs), the NPs were characterized and their ability to induce efficient immune responses against B. pertussis was studied in a mouse model. Our findings showed that PTd + FHA-loaded TMC NPs have strong ability to induce IL-4, IL-17, IFN-γ, IgG, and IgA in the mouse model. Results from this study suggest that nasal administration of the PTd + FHA-loaded TMC NPs induced not only a systemic immune response but also a local mucosal response, which may improve the efficacy of pertussis prevention through respiratory tract transmission.

The mucosal immune system serves as the first line of defense against Bordetella pertussis. Intranasal vaccination, due to its potential to induce systemic and mucosal immune responses, appears to prevent the initial adherence and colonization of the bacteria at the first point of contact. In the present study, two B. pertussis antigens, pertussis Toxoid (PTd) and Filamentous hemagglutinin (FHA), which play a very significant role in virulence and protection against pertussis, were encapsulate into N-trimethyl chitosan (TMC) nanoparticulate systems. After preparation of TMC nanoparticles (NPs), the NPs were characterized and their ability to induce efficient immune responses against B. pertussis was studied in a mouse model. Our findings showed that PTd + FHA-loaded TMC NPs have strong ability to induce IL-4, IL-17, IFN-γ, IgG, and IgA in the mouse model. Results from this study suggest that nasal administration of the PTd + FHA-loaded TMC NPs induced not only a systemic immune response but also a local mucosal response, which may improve the efficacy of pertussis prevention through respiratory tract transmission.

5.65.6

Antibody measurements:

For PT-specific IgG ELISA, plates were coated with 0.5 µg/mL purified PT (List Biologicals Campbell, California) in PBS and the assay performed as previously described [133]. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

Antibody measurements:

For PT-specific IgG ELISA, plates were coated with 0.5 µg/mL purified PT (List Biologicals Campbell, California) in PBS and the assay performed as previously described [133]. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

https://archive.hshsl.umaryland.edu/handle/10713/9580https://archive.hshsl.umaryland.edu/handle/10713/95802019-12-012019-12-0110.1080/21691401.2019.162994810.1080/21691401.2019.1629948Pertussis Toxin from B. pertussis, Lyophilized in BufferPertussis Toxin from B. pertussis, Lyophilized in BufferMary.Y.Masterson@gmail.comMary.Y.Masterson@gmail.com133;Antibody;Assay;B. pertussis;Bacteria;Bordetella pertussis;Colonization;Efficacy;ELISA;FHA;Filamentous Hemagglutinin;Hemagglutinin;IgA;IgG;IL;Immune response;Immune System;Induce;Intranasal;List;Mouse;Mucosal;Nasal;Novel;Pertussis;Preparation;Prevention;Purified;Respiratory;Response;Specific;Study;Toxoid;Transmission;Vaccination;Vaccine;Virulence;Artificial Cells, Nanomedicine, And Biotechnology133;Antibody;Assay;B. pertussis;Bacteria;Bordetella pertussis;Colonization;Efficacy;ELISA;FHA;Filamentous Hemagglutinin;Hemagglutinin;IgA;IgG;IL;Immune response;Immune System;Induce;Intranasal;List;Mouse;Mucosal;Nasal;Novel;Pertussis;Preparation;Prevention;Purified;Respiratory;Response;Specific;Study;Toxoid;Transmission;Vaccination;Vaccine;Virulence;Artificial Cells, Nanomedicine, And Biotechnology180180bordetella-pertussis-antigens-encapsulated-intobordetella-pertussis-antigens-encapsulated-into