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March 15, 2024
Iscience
Moe, AAK;Bautista, TG;Trewella, MW;Korim, WS;Yao, ST;Behrens, R;Driessen, AK;McGovern, AE;Mazzone, SB;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
Retrograde viral tracing from the trachea and the lungs
… In the subsequent confirmatory experiments, the Cre-dependent viral tracer, AAVrg-CAG-FLEX-tdTomato-WPRE10GH, was injected into the trachea of heterozygous CGRP-Cre mice (n = 2). Again, animals were anaesthetized with isoflurane (4% for induction and 1.8–2.5% for maintenance), a midline incision was made in the neck and the muscles overlying the trachea were retracted to expose the trachea. Next using a Hamilton syringe, 1 μl of AAVrg-CAG-FLEX-tdTomato-WPRE10GH together with 2μl of saline was injected into the lumen of the trachea. After 4 weeks, the animals were euthanized and their brains, trachea and vagal ganglia were collected for immunohistochemistry. Alternatively, cholera toxin B (CTB, catalogue number #104, List Biological Labs, 0.3% w/v, 5 μl per injection), a retrograde tracer, was injected into the trachea of Phox2b-Cre;loxP-ChR2 mice (n = 6) following the experimental procedures described above. After one week, the animals were euthanized and their brains and vagal ganglia were collected for immunohistochemistry.
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
March 1, 2024
Cell Reports
Koller, B;Nguyen, M;Snouwaert, J;Gabel, C;Ting, J;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
… Anthrax lethal toxin List Biological Laboratories Inc. #171B/#172B …
Treatments
See Table S1 for list of Figures/panels in each figure and corresponding dose and exposure time. General information for the methods is provided here. To induce cytokine release from macrophages, microglia and BMDM cells were primed for 4–5 h (or time indicated in Table S1)with 1 μg/mL of LPS in culture medium, and some cells were pre-treated with 1 μM of CP456773/MCC950 for 30 min before ATP stimulation. Cells were then pulsed with 5 mM ATP for 30 min. For cytokine induction by anthrax lethal toxin (LeTx), LPS-primed cells were treated with LeTx at a final concentration of 250 ng/mL (LF and PA) for 3 h. Cell supernatants were collected and frozen at −80°C until assayed.
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
March 1, 2024
Cell Reports
Chu, L;Qian, L;Chen, Y;Duan, S;Ding, M;Sun, W;Meng, W;Zhu, J;Wang, Q;Hao, H;Wang, C;Cui, S;
Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
March 1, 2024
Nature Immunology
Linnerbauer, M;Lößlein, L;Vandrey, O;Peter, A;Han, Y;Tsaktanis, T;Wogram, E;Needhamsen, M;Kular, L;Nagel, L;Zissler, J;Andert, M;Meszaros, L;Hanspach, J;Zuber, F;Naumann, UJ;Diebold, M;Wheeler, MA;Beyer, T;Nirschl, L;Cirac, A;Laun, FB;Günther, C;Winkler, J;Bäuerle, T;Jagodic, M;Hemmer, B;Prinz, M;Quintana, FJ;Rothhammer, V;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
February 29, 2024
Biorxiv
Goldstein, N;Maes, A;Allen, H;Nelson, T;Kruger, K;Kindel, M;Smith, N;Carty, J;Villari, R;Cho, E;Marble, E;Khanna, R;Taylor, B;Kennedy, A;Betley, J;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
February 27, 2024
Biorxiv
Upadhyay, A;Gradwell, M;Vajtay, T;Conner, J;Sayal, A;Azadegan, C;Patel, K;Thackary, J;Bohic, M;Imai, F;Ogundare, S;Yoshida, Y;Abdus-Saboor, I;Azim, E;Abraira, V;
Product: Anti-Cholera Toxin B Subunit (Goat)
February 27, 2024
Cell Reports
Cheng, M;Li, J;Song, J;Song, H;Chen, Y;Tang, H;Wei, H;Sun, R;Tian, Z;Wang, X;Peng, H;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
February 27, 2024
Nature Communications
Jo-Watanabe, A;Inaba, T;Osada, T;Hashimoto, R;Nishizawa, T;Okuno, T;Ihara, S;Touhara, K;Hattori, N;Oh-Hora, M;Nureki, O;Yokomizo, T;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
February 23, 2024
Infection And Immunity
Choi, M;Shridhar, S;Fox, H;Luo, K;Amin, MN;Tennant, SM;Simon, R;Cross, AS;
Product: LPS from Escherichia coli O111:B4
February 23, 2024
Frontiers In Immunology
De Weerdt, L;Thiriard, A;Leuridan, E;Marchant, A;Maertens, K;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer