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Page 695 out of 728

Divergent requirement for Gαs and cAMP in the differentiation and inflammatory profile of distinct mouse Th subsets

Li, X;Murray, F;Koide, N;Goldstone, J;Dann, SM;Chen, J;Bertin, S;Fu, G;Weinstein, LS;Chen, M;Corr, M;Eckmann, L;Insel, PA;Raz, E;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Oral CT/OVA immunization:

Mice were immunized orally by gavage on day 0 and day 14 with CT (20 μg, List Laboratories) and OVA (200 μg, Sigma-Aldrich), as described previously (7). On day 39, the mice were challenged with OVA (200 μg i.p.). Single-cell suspensions from spleens were collected on day 42 and incubated for 3 days with medium alone or supplemented with OVA (200 μg/ml). The concentration of cytokines in the supernatants was determined by ELISA.

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

PubMed ID: 22326954

Macrophages participate in IL-17-mediated inflammation

Barin, JG;Baldeviano, GC;Talor, MV;Wu, L;Ong, S;Quader, F;Chen, P;Zheng, D;Caturegli, P;Rose, NR;Ciháková, D;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction of experimental autoimmune myocarditis:

Previously well-established methods were used to induce EAM [14]. Briefly,100 μg commercially fMOC synthesized MyHCα614–629 [15] was emulsified 1:1 in CFA (Sigma, St. Louis, MO) supplemented with 5 mg/mL of heat-killed M. tb strain H37Ra (Difco, Detroit, MI), and installed sc in mice twice on days 0 and 7. On day 0, mice also received 500 ng of pertussis toxin ip (List Biologicals, Campbell, CA). ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22161142

Oxytocin-immunoreactive innervation of identified neurons in the rat dorsal vagal complex

Llewellyn-Smith, IJ;Kellett, DO;Jordan, D;Browning, KN;Travagli, RA;
Product: Anti-Cholera Toxin B Subunit (Goat)

Immunohistochemistry - Primary Antibodies:

... Cholera toxin B subunit, B subunit (choleragenoid) of cholera toxin isolated from Vibrio cholerae type Inaba 569B, List Biological Laboratories (Campbell, CA, USA), goat polyclonal, Catalog # 703; ...

PubMed ID: 22188490

Regulation of autoimmune neuroinflammation by stress-responsive genes

Cunha, A;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

PTx (List Biological Laboratories) was dissolved in PBS (100 ng/l).

Experimental autoimmune encephalomyelitis (EAE):

... PTx facilitates T cell access to the CNS, presumably via TLR4-mediated induction of adhesion molecules in the endothelium of the blood-brain barrier84. In addition, PTx contributes to the break of T cell tolerance, promoting clonal expansion and cytokine production by T cells79,80. This immunization model promotes the activation of TH cells, specifically of the TH1 and TH17 lineages79,80. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

 

Restricted replication of xenotropic murine leukemia virus-related virus in pigtailed macaques

Del Prete, GQ;Kearney, MF;Spindler, J;Wiegand, A;Chertova, E;Roser, JD;Estes, JD;Hao, XP;Trubey, CM;Lara, A;Lee, K;Chaipan, C;Bess, JW;Nagashima, K;Keele, BF;Macallister, R;Smedley, J;Pathak, VK;Kewalramani, VN;Coffin, JM;Lifson, JD;
Product: Tetanus Toxoid from Clostridium tetani

Flow cytometry:

... Medium alone and 10  g/ml tetanus toxoid protein (List Biological Laboratories, Inc.) were used as negative controls, ...

PubMed ID: 22238316