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Page 693 out of 693

The Basolateral Amygdala and Superior Colliculus Regulate Physiological and Behavioral Measures of Avoidance and Approach

Elorette, C;
Product: Cholera Toxin B Subunit (Choleragenoid)from Vibrio cholerae in Low Salt

Subjects:

Two male macaque monkeys (1 Macaca mulatta (Y), 1 Macaca nemestrina (S)) were used for these experiments. Animal Y was 4 years old and weighed 8.2 kg and Animal S was 6 years old and weighed 17.4 kg at the time of surgery. The subjects used in this study were anatomically typical, with no observable abnormalities. MRI-guided injections of anatomical tracers were performed stereotaxically under sterile conditions. Injection sites, tracers used, and volumes of injection for each case are shown in Table 2.1. The animals received bilateral injections of anterograde tracer (fluoroemerald; FE) in superior colliculus and retrograde tracer (choleratoxin B; CTB) in the lateral nucleus of the amygdala; this yielded 4 hemispheres for anatomical analysis.

Table 2.2

Choleratoxin B List Labs

 

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 μl of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA, 0.003 M NaN3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

Tumor Necrosis Factor Receptor 2 Promotes Neuroprotection During Chronic Autoimmune Neuroinflammation

Gomez-Rivera, F;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

... Mice also received intraperitoneal (ip) injections of 200 ng Bordetella pertussis toxin (List Biological, Campbell, CA) on days 0 and 2. For induction of passive EAE by adoptive transfer, donor mice were immunized sc with 200 µg of MOG35-55 in CFA ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

β3 human papillomavirus species share several biological properties with high risk mucosal types

Minoni, L;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Biological Material:

...Cholera Toxin (FAD)

8,3 ng/ml (working concentration)

List Biological laboratories, INC (Company)

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

Oxysterol Control of Macrophage-dependent Inflammation

Van Dang, E;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

... Pertussis toxin (List Biological Labs) in 100 ul saline was injected. intravenously twice, on days 0 and 2, and disease severity was assigned scores. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).