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Page 693 out of 728

Bystander-mediated stimulation of proteolipid protein-specific regulatory T (Treg) cells confers protection against experimental autoimmune encephalomyelitis (EAE) via TGF-β

Jun, S;Ochoa-Repáraz, J;Zlotkowska, D;Hoyt, T;Pascual, DW;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Adoptive transfer, EAE challenge, and TGF-β neutralization:

To isolate Treg cells, CD4+CD25 and CD4+CD25+ T cells were obtained from spleens and head and neck lymph nodes (HNLNs) from H696- or H647-vaccinated mice. ...

To induce EAE, SJL females were given s.c 200 μg of the encephalitogenic PLP peptide (PLP139–151; HSLGKWLGHPDKF, HPLC-purified to >90%; Biosynthesis, Inc., Lewisville, TX) in 200 μl of complete Freund’s adjuvant. On days 0 and 2, mice received i.p. 200 ng of Bordetella pertussis toxin (List Biological Laboratories; Campbell, CA), as previously described ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

Annotator: postprocessing software for generating function-based signatures from quantitative mass spectrometry

Sylvester, JE;Bray, TS;Kron, SJ;
Product: LPS from Escherichia coli O111:B4

Sample Preparation:

To provide a source of data that was rich in content for functional annotation, we prepared a complex sample of soluble proteins from neutrophils activated by lipopolysaccharide (LPS). The human promyelocytic HL-60 cell line (ATCC) was differentiated in culture using 1 μM alltrans retinoic acid (Sigma-Aldrich), 6 pM 1α,25-Dihydroxyvitamin D3 (Sigma-Aldrich), and 30 ng/mL granulocyte-colony stimulatory factor (Invitrogen) in Iscove's Modified Dulbecco's medium (Mediatech, Inc.) supplemented with 20% FBS and 4 mM L-glutamine.31 Cells were activated via treatment with 100 ng/mL of lipopolysaccharide (LPS) from E. coli O111:B4 (List Biological Laboratories) for 30 minutes. ...

PubMed ID: 22224429

Antibodies against a Plasmodium falciparum antigen PfMSPDBL1 inhibit merozoite invasion into human erythrocytes

Sakamoto, H;Takeo, S;Maier, AG;Sattabongkot, J;Cowman, AF;Tsuboi, T;
Product: Tetanolysin from Clostridium tetani

... To harvest parasite pellets, mature schizonts were purified using Percoll (GE Healthcare, Camarillo, CA) density gradient centrifugation and further treated with tetanolysin (List Biological Laboratories, Campbell, CA), washed with phosphate buffered saline (PBS) containing ...

PubMed ID: 22248820

pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen

Matsumoto, Y;Zhang, Q;Akita, K;Nakada, H;Hamamura, K;Tokuda, N;Tsuchida, A;Matsubara, T;Hori, T;Okajima, T;Furukawa, K;Urano, T;Furukawa, K;
Product: Cholera Toxin B Subunit Biotin Conjugate

... Cholera toxin B (CTB) subunit-biotin conjugates was from List Biological Laboratories, Inc. (Campbell, CA). ...

PubMed ID: 22306014

Proteomic identification of immunoproteasome accumulation in formalin-fixed rodent spinal cords with experimental autoimmune encephalomyelitis

Jain, MR;Li, Q;Liu, T;Rinaggio, J;Ketkar, A;Tournier, V;Madura, K;Elkabes, S;Li, H;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE induction, disability evaluation and tissue harvesting:

... For the mouse model, 8–10 weeks-old female C57BL/6 mice (Taconic, Hudson, NY) were immunized subcutaneously in the flank with 200 µg myelin oligodendrocyte glycoprotein peptide 35–55 (MEVGWYRSPFSRVVHLYRNGK; Stanford PAN Facility, Stanford, CA) emulsified in CFA that had been supplemented with M. tuberculosis strain H37RA (MT; Difco Laboratories, Detroit, MI) at 7 mg/ml. All mice also received an intraperitoneal injection of 350 ng pertussis toxin (List Biological, Campbell, CA) at 0 and 48 h post-immunization. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22188123