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Page 692 out of 728

Maximal Adjuvant Activity of Nasally Delivered IL-1a Requires Adjuvant-Responsive CD11c+ Cells and Does Not Correlate with Adjuvant-Induced In Vivo Cytokine Production

Thompson, AL;Johnson, BT;Sempowski, GD;Gunn, MD;Hou, B;DeFranco, AL;Staats, HF;
Product: Anthrax Lethal Factor (LF),Recombinant from B. anthracis

Nasal vaccination:

Mice were anesthetized with isoflurane before vaccination. Vaccines were administered intranasally in 10 ml (5ml per nostril). Nonchimeric mice were vaccinated on days 0, 7, and 21. Chimeric mice were vaccinated on days 0, 7, 21, and 42. For each chimeric study replicate, mice were divided into groups of 4 to 8. Vaccine groups included PBS alone, recombinant LF (rLF) alone (#172; List Biological Laboratories Cambell, CA; 4 endotoxin units [EU]/mg), rLF + 4 mg IL-1a (400-ML; 0.00144 EU/mg; R&D, Minneapolis, MN), and rLF + 1 mg cholera toxin (CT) (EU/mg unknown; List #100B). For three of the four Il1r1 chimeric mouse experiments, mice were vaccinated with 25 mg LF for the day 0, 7, and 21 immunizations and 50 mg LF on day 42. For the remaining experiment, mice were immunized with 50 mg LF each time. The calculated endotoxin delivery per mouse was ∼0.20 (LF alone) to 0.22 EU (LF + IL-1a). Data from each group for all repeat experiments were combined and analyzed together. Mice vaccinated with LF + CT served as a positive control, and LF + CT-vaccinated mice responded similarly in all four chimeric groups.

Maximal adjuvant activity of nasally delivered IL-1α requires adjuvant-responsive CD11c(+) cells and does not correlate with adjuvant-induced in vivo cytokine production

Thompson, AL;Johnson, BT;Sempowski, GD;Gunn, MD;Hou, B;DeFranco, AL;Staats, HF;
Product: Anthrax Lethal Factor (LF),Recombinant from B. anthracis

Nasal vaccination:

Mice were anesthetized with isoflurane before vaccination. Vaccines were administered intranasally in 10 ml (5ml per nostril). Nonchimeric mice were vaccinated on days 0, 7, and 21. Chimeric mice were vaccinated on days 0, 7, 21, and 42. For each chimeric study replicate, mice were divided into groups of 4 to 8. Vaccine groups included PBS alone, recombinant LF (rLF) alone (#172; List Biological Laboratories Cambell, CA; 4 endotoxin units [EU]/mg), rLF + 4 mg IL-1a (400-ML; 0.00144 EU/mg; R&D, Minneapolis, MN), and rLF + 1 mg cholera toxin (CT) (EU/mg unknown; List #100B). For three of the four Il1r1 chimeric mouse experiments, mice were vaccinated with 25 mg LF for the day 0, 7, and 21 immunizations and 50 mg LF on day 42. For the remaining experiment, mice were immunized with 50 mg LF each time. The calculated endotoxin delivery per mouse was ∼0.20 (LF alone) to 0.22 EU (LF + IL-1a). Data from each group for all repeat experiments were combined and analyzed together. Mice vaccinated with LF + CT served as a positive control, and LF + CT-vaccinated mice responded similarly in all four chimeric groups. ...

PubMed ID: 22345651

Spatiotemporal inhibition of innate immunity signaling by the Tbc1d23 RAB-GAP

De Arras, L;Yang, IV;Lackford, B;Riches, DW;Prekeris, R;Freedman, JH;Schwartz, DA;Alper, S;
Product: LPS from Escherichia coli O111:B4

... PAMP exposures were performed for 6 h, except in the time-course experiments. PAMPs used for BMDM and cell line exposures were from InvivoGen, except for E. coli O111:B4 LPS, which was from List Biological Laboratories. ...

PubMed ID: 22312129

Species-specific recognition of Aspergillus fumigatus by Toll-like receptor 1 and Toll-like receptor 6

Rubino, I;Coste, A;Le Roy, D;Roger, T;Jaton, K;Boeckh, M;Monod, M;Latgé, JP;Calandra, T;Bochud, PY;
Product: ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Cells and Reagents:

... Stimulations were performed using 100 ng/mL of Salmonella minnesota ultra pure lipopolysaccaride (LPS) (List Biological Laboratories), ...

PubMed ID: 22315281

Deciphering a neuronal circuit that mediates appetite

Wu, Q;Clark, MS;Palmiter, RD;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animal maintenance and neuron ablation

... All except Tph2lox/lox mice were on the C57Bl/6 background (> 9 generations backcrossed); Tph2lox/lox mice were on a mixed 129/Sv x C57Bl/6 background. AgrpDTR/DTR male mice were bred with Slc17a6lox/lox female mice to generate AgrpDTR/+; Slc17a6lox/+ mice, which were further bred to each other to create AgrpDTR/+; Slc17a6lox/lox mice and AgrpDTR/+; Slc17a6lox/+ control mice. Similar breeding strategy was adopted when generating AgrpDTR/+; Tph2lox/lox mice and AgrpDTR/+; Grin1lox/lox mice. Mice were group housed with standard chow diet (LabDiet 5053) and water provided ad libitum until the beginning of the experiments. All experiments were performed with ~8-wk-old male mice. To ablate AgRP neurons, mice carrying the AgrpDTR allele, were injected twice with DT (50 μg/kg; intramuscular; 2 d apart; List Biological Laboratories, Campbell, CA)4. The extent of ablation (>95%) was determined by immunohistochemistry47.

PubMed ID: 22419158