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Page 663 out of 728

Chitosan oligosaccharide as potential therapy of inflammatory bowel disease: therapeutic efficacy and possible mechanisms of action

Yousef, M;Pichyangkura, R;Soodvilai, S;Chatsudthipong, V;Muanprasat, C;

... For measurements of cytokine release in T84 cells, T84 cell monolayers were stimulated apically with serum-free medium containing LPS (10 μg/ml; List Biological Laboratories, CA, USA), and basolaterally with TNF-α ...

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://www.listlabs.com/product-information/lipopolysaccharides/

PubMed ID: 22475725

CNS-specific therapy for ongoing EAE by silencing IL-17 pathway in astrocytes

Yan, Y;Ding, X;Li, K;Ciric, B;Wu, S;Xu, H;Gran, B;Rostami, A;Zhang, GX;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction and assessment of EAE:

For active EAE, mice were immunized subcutaneously on the back with 200 µg of MOG35-55 (MEVGWYRSPFSRVVHLYRNGK) emulsified in CFA (Difco Lab, Detroit, MI) containing 4 mg/ml Mycobacterium tuberculosis H37Ra (Difco). Two hundred nanogram of pertussis toxin (List Biological Lab, Epsom, England) was given intraperitoneally on days 0 and 2 (p.i.). ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22434134

Effects of an aqueous extract of North American ginseng on MOG(35-55)-induced EAE in mice

Bowie, LE;Roscoe, WA;Lui, EM;Smith, R;Karlik, SJ;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE:

... Mice also received a co-injection of 500 µL Bordetella pertussis toxin (List Biological Laboratories, Cedarlane, Hornby, Ontario) on day 0 and day 2 post-immunization. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22720838

Elafin in Intestinal Barrier Fortification: A Potential Adjuvant Therapy for Gluten Intolerance

Wiepjes, MC;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Mucosal sensitization was achieved via intragastric gavage of 25 µg of cholera toxin (List Biological Laboratories, CA, USA) and 500 µg of peptic-tryptic-digest of gliadin (PT-gliadin) dissolved in 0.02 M acetic acid. ...

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

Estrogen treatment prevents gray matter atrophy in experimental autoimmune encephalomyelitis

MacKenzie-Graham, AJ;Rinek, GA;Avedisian, A;Morales, LB;Umeda, E;Boulat, B;Jacobs, RE;Toga, AW;Voskuhl, RR;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Experimental Autoimmune Encephalomyelitis:

Mice were immunized with MOG peptide 35–55 (300 µg/mouse) and Mycobacterium tuberculosis (500 µg/mouse) emulsified in Complete Freund’s Adjuvant subcutaneously, in a volume of 0.1 ml/mouse over two sites: the right draining inguinal and axillary lymph nodes. One week later a booster immunization was delivered subcutaneously, over the contralateral draining lymph nodes. Pertussis toxin (500 ng/mouse) (List Biological Laboratories, Inc., Campbell, CA) was injected intraperitoneally on days 0 and 2 ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22411609