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Page 660 out of 728

The T cell response to IL-10 alters cellular dynamics and paradoxically promotes central nervous system autoimmunity

Liu, X;Alli, R;Steeves, M;Nguyen, P;Vogel, P;Geiger, TL;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE induction and clinical evaluation:

EAE was induced by s.c. immunization with 100 mg MOG35–55 peptide in 100 ml CFA containing 0.4 mg Mycobacterium tuberculosis H37RA (Difco); 200 ng pertussis toxin (List Biological Laboratories) was administered i.v. on days 0 and 2. Clinical scoring was: 1 = limp tail; 2 = hind limb paresis or partial paralysis; 3 = total hind limb paralysis; 4 = hind limb paralysis and body or front limb paresis or paralysis; 5 = moribund.

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22711892

Experimental autoimmune encephalomyelitis

Rao, P;Segal, BM;
Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

... Pertussis toxin (salt-free; List Biological Laboratories, Campbell, CA). It is sold as a lyophilized powder. Reconstitute with PBS to a final concentration of 2 μg/ml and store at 4 °C. 10. ...

Mechanism for p38α-mediated experimental autoimmune encephalomyelitis

Namiki, K;Matsunaga, H;Yoshioka, K;Tanaka, K;Murata, K;Ishida, J;Sakairi, A;Kim, J;Tokuhara, N;Shibakawa, N;Shimizu, M;Wada, Y;Tokunaga, Y;Shigetomi, M;Hagihara, M;Kimura, S;Sudo, T;Fukamizu, A;Kasuya, Y;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction and Assessment of EAE:

For the induction of EAE, mice were immunized subcutaneously in the four flanks with 100 μg of MOG(35–55) emulsified 1:1 in complete Freund's adjuvant (Difco) supplemented with 500 μg of Mycobacterium tuberculosis H37Ra on day 0. In addition, 120 ng of pertussis toxin (PTX; List Biological Laboratories, Campbell, CA) was injected intravenously on days 0 and 2. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22637476

Rodent models of experimental autoimmune uveitis

Agarwal, RK;Silver, PB;Caspi, RR;
Product: Pertussis Toxin from B. pertussis (in Glycerol)

Material:

... Pertussis Toxin (Catalog #179A; List Biologicals, Campbell, CA). ...

The CCL2 synthesis inhibitor bindarit targets cells of the neurovascular unit, and suppresses experimental autoimmune encephalomyelitis

Ge, S;Shrestha, B;Paul, D;Keating, C;Cone, R;Guglielmotti, A;Pachter, JS;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Treatment of animals:

... For active induction of EAE, C57BL/6 female mice were immunized with MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK) of rat origin, by a modification of the method previously described ... Mice were also injected i.p. with 200 ng pertussis toxin (List Laboratories, Campbell CA, USA) in PBS on days 0 and 2 following the first immunization. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22788993