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Page 658 out of 728

Suppression of TRIF-dependent signaling pathway of toll-like receptors by allyl isothiocyanate in RAW 264.7 macrophages

Kim, SJ;Park, HJ;Shin, HJ;Shon, DH;Kim, DH;Youn, HS;

... Purified LPS was purchased from List Biologicals (San Jose, CA, USA). ...

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://www.listlabs.com/product-information/lipopolysaccharides/

PubMed ID: 22668719

Syndecan-4 regulates early neutrophil migration and pulmonary inflammation in response to lipopolysaccharide

Tanino, Y;Chang, MY;Wang, X;Gill, SE;Skerrett, S;McGuire, JK;Sato, S;Nikaido, T;Kojima, T;Munakata, M;Mongovin, S;Parks, WC;Martin, TR;Wight, TN;Frevert, CW;
Product: LPS from Escherichia coli O111:B4

Reagents:

The reagents used in this study were Escherichia coli serotype 0111:B4 LPS (List Biological Laboratories, Campell, CA ...

Cell Culture:

Bone marrow–derived macrophages (BMDMs) were cultured in macrophage medium (RPMI 1640, 10% FBS, 30% L929 cell supernatant, 2 mM l-glutamine, 100 IU/mL penicillin, and 100 μg/ml streptomycin) as described (20). After being cultured in macrophage medium for 6 days, BMDMs were isolated, counted, and cultured in macrophage media for 24 hours and then stimulated with LPS (10 or 100 ng/ml), IL-4/IL-13 (10 ng/ml), IL-10 (10 ng/ml), or RPMI 1640 for up to 48 hours. Alveolar macrophages isolated with repeated BAL using PBS were cultured for 24 hours in macrophage media and then stimulated with LPS for 4 hours. BEAS-2B cells were cultured in RPMI 1640 supplemented with 10% BSA, penicillin, and streptomycin for 5 to 6 days until they reached 90% confluence. The culture medium was then replaced, and low-molecular-weight heparin, syndecan-4, or RPMI 1640 was added for 1 hour. Cells were washed with PBS and incubated with LPS (1,000 ng/ml), TNF-α (1 ng/ml), or RPMI 1640 without heparin or syndecan-4 for 3 hours. Mouse tracheal air–liquid interface cultures were performed as described and stimulated with LPS or RPMI 1640 for 4 and 24 hours (33). Supernatants were removed and RNA was harvested.

 

PubMed ID: 22427536

Transcriptional regulator Id2 is required for the CD4 T cell immune response in the development of experimental autoimmune encephalomyelitis

Lin, YY;Jones-Mason, ME;Inoue, M;Lasorella, A;Iavarone, A;Li, QJ;Shinohara, ML;Zhuang, Y;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction of EAE:

EAE was induced in 6-wk-old male mice by s.c. injection with 100 mg myelin oligodendrocyte glycoprotein (MOG)35–55 peptide (MEVGWYRSPFSRVVHLYRNGK; United Peptide, Rockville, MD) emulsified in 100 ml CFA (Sigma-Aldrich, St. Louis, MO) containing 2 mg/ml heatkilled Mycobacterium tuberculosis H37RA (Difco, Detroit, MI). Mice were also injected i.p. with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) on day 0 and day 2. Clinical signs of EAE were recorded daily using a 0–4 scoring system: 0, normal; 1, limp tail; 2, unsteady gait; 3, hind-limb paralysis; 4, fore-limb paralysis.

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22745378

Identification of exosite-targeting inhibitors of anthrax lethal factor by high-throughput screening

Bannwarth, L;Goldberg, AB;Chen, C;Turk, BE;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Materials:

Recombinant LF and PA were from List Biological Laboratories. ...

SDS-PAGE-Based Cleavage Assay:

LF (25 nM in assay buffer) was mixed with compound at the indicated concentration, and the reaction was initiated by adding unlabeled MKK6 to 1 μM and stopped after 30 min at RT by adding 4× SDS-PAGE loading buffer. ...

Cytotoxicity Assay:

RAW264.7 cells were plated in 96-well dishes at 4 × 105 cells per well and were allowed to recover for 16 hr, after which the medium was removed and replaced with fresh complete medium (100 μl per well) containing 400 μM compound or 4% DMSO vehicle control. After 30 min, PA (0.5 μg/ml) and/or LF (0.001–0.3 μg/ml) were added and incubation continued for an additional 4 hr.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

PubMed ID: 22840775

SLAT/Def6 plays a critical role in the pathogenic process of experimental autoimmune uveitis (EAU)

Vistica, BP;Shi, G;Nugent, L;Tan, C;Altman, A;Gery, I;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction and evaluation of EAU:

SLAT/Def6 KO mice and their C57BL/6 controls were immunized with bovine IRBP (150 μg), emulsified in complete Freund’s adjuvant (CFA), administered subcutaneously [10]. In addition, the mice were injected intraperitoneally with 0.2 μg pertussis toxin (List Biological Laboratories, Inc., Campbell, CA). The development of ocular inflammation was determined by fundoscopy on day 12 post-immunization and by histological examination on day 14, following euthanization. Severity of disease, on a scale of 0–4, in half point increments, was scored as detailed elsewhere [11,12].

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22815639