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Page 639 out of 693

Organization of the auditory brainstem in a lizard, Gekko gecko. I. Auditory nerve, cochlear nuclei, and superior olivary nuclei

Tang, Y;Christensen-Dalsgaard, J;Carr, CE;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Tract tracing and rapid Golgi:

When physiological recordings were complete, the tungsten microelectrode was replaced by a tracer-filled glass pipette, through which recordings of auditory responses could be made prior to iontophoresis of neural tract tracers. Three different tracers were used, neurobiotin (NB; Molecular Probes, Eugene, OR), cholera toxin (CTX; List Biological Laboratories, Campbell, CA), ...

For tract tracing with CTX, immunohistochemical procedures were used to visualize the labeled terminals and fibers. Free-floating sections (40 μm) were preincubated for 1 hour in a blocking solution consisting of 10% normal goat serum and 0.3% Triton X-100 in 0.1 M PBS (pH 7.4). Subsequently, sections were incubated with goat anticholeragenoid antibody (List Biological Laboratories), diluted 1:10,000 overnight at 4°C. After multiple washes in PBS, sections were incubated for 1 hour in biotinylated secondary antiserum diluted 1:1,500. ...

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

PubMed ID: 22120438

Protease-activated receptor 1 (PAR1) coupling to G(q/11) but not to G(i/o) or G(12/13) is mediated by discrete amino acids within the receptor second intracellular loop.

McCoy, KL;Gyoneva, S;Vellano, CP;Smrcka, AV;Traynelis, SF;Hepler, JR;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Materials:

... Pertussis toxin from List Biologicals (Campbell, CA); ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22306780

Protection from bacterial-toxin-induced apoptosis in macrophages requires the lipogenic transcription factor sterol regulatory element binding protein 1a

Im, SS;Osborne, TF;
Product: Alpha Toxin from Staphylococcus aureus

Reagents:

Alpha-toxin from Staphylococcus aureus was purchased from List Biological Laboratories (Campbell, CA). ...

Results:

Alpha-toxin-mediated apoptosis is increased in SREBP-1aDF macrophages.We recently demonstrated that SREBP-1aDF mice have an impaired proinflammatory response in vivo and in isolated macrophages in culture (8). To investigate the role of SREBP-1a in other critical functions of the innate immune response, we compared the sensitivities of bone marrow-derived macrophages (BMDM) from WT and SREBP-1aDF mice to a classic bacterial pore-forming alpha-toxin from S. aureus (2). ...

Product #120, Alpha Toxin from Staphylococcus aureus, has been discontinued.  Large quantities may be obtained through Bulk Requests and Custom Orders.

PubMed ID: 22493063

PTPN22 alters the development of regulatory T cells in the thymus

Maine, CJ;Hamilton-Williams, EE;Cheung, J;Stanford, SM;Bottini, N;Wicker, LS;Sherman, LA;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Experimental autoimmune encephalomyelitis induction:

Female mice, at least 9 wk old, were injected s.c. with 200 mg myelin oligodendrocyte protein (MOG) 35–55 (Genscript, Piscataway, NJ) in CFA (Difco, Detroit, MI), with half of the mice injected behind the neck and the other half at the base of the tail. Two hours later, mice were injected i.p. with 300 ng pertussis toxin (List Biologicals, Campbell, CA), and another injection was given 24 h later. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22539785

Resveratrol neuroprotection in a chronic mouse model of multiple sclerosis

Fonseca-Kelly, Z;Nassrallah, M;Uribe, J;Khan, RS;Dine, K;Dutt, M;Shindler, KS;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction and Scoring of EAE:

Experimental autoimmune encephalomyelitis was induced as in our prior studies (Quinn et al., 2011). ... Control mice were injected with an equal volume of phosphate buffered saline (PBS) and CFA. EAE and control mice received 200 ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1 ml PBS by i.p. injection on the day of immunization (day 0) and again on day 2. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22654783