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Page 639 out of 659

Epigallocatechin-3-gallate ameliorates experimental autoimmune encephalomyelitis by altering balance among CD4+ T-cell subsets

Wang, J;Ren, Z;Xu, Y;Xiao, S;Meydani, SN;Wu, D;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction and Evaluation of EAE:

Mice were immunized s.c. in the flanks with 200 μg of myelin oligodendrocyte glycoprotein (MOG)35-55 peptide (synthesized by the Tufts University Core Facility Laboratory) in a 200-μL emulsion of complete freund's adjuvant (CFA) containing 5 mg/mL heat-killed Mycobacterium tuberculosis H37Ra extract (Sigma-Aldrich, St. Louis, MO) on day 0 and i.p. injected with 200 ng per mouse of pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2. The clinical symptoms were scored daily from day 0 to 30 after immunization, as follows: ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22056360

Glycoconjugates prevent B. anthracis toxin-induced cell death through binding while activating macrophages

Tarasenko, O;Scott, A;Soderberg, L;Alusta, P;
Product: Anti-Protective Antigen from B. anthracis (Goat)

... Recombinant PA, EF, LF toxins and goat anti-PA primary antibody (Ab) were obtained from the List Biological Laboratories, Inc. ... 

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

PubMed ID: 22134879

Human foetal intestinal fibroblasts are hyper-responsive to lipopolysaccharide stimulation

Okogbule-Wonodi, AC;Li, G;Anand, B;Luzina, IG;Atamas, SP;Blanchard, T;
Product: ULTRA PURE LPS from Escherichia coli O111:B4

Reagents:

... An ultrapurified form of E. coli 0111:B4 LPS was purchased from List Biological Lab. Inc. (Campbell, CA). ...

PubMed ID: 21956143

Identification of viral determinants of murine norovirus pathogenesis

Strong, D;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

... Each well of a high binding microtiter plate (Thermo Fisher Scientific, Waltham, MA) was coated with 0.25 µg of cholera toxin (List Biological Laboratories, Campbell, CA) diluted in coating buffer over night at 4°C ...

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

Immunoregulation of experimental autoimmune encephalomyelitis by the selective CB1 receptor antagonist

Lou, ZY;Zhao, CB;Xiao, BG;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE:

... supplemented with 3 mg/ml M. tuberculosis H37Ra (BD Difco, Detroit, MI; 400 μg/mice). Mice were injected with 400 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) via a tail vein at the same time of immunization and again 48 hrs later. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 21922514