Citations

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4694 citations found

Investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomy

Moe, AAK;Bautista, TG;Trewella, MW;Korim, WS;Yao, ST;Behrens, R;Driessen, AK;McGovern, AE;Mazzone, SB;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • Retrograde viral tracing from the trachea and the lungs
    … In the subsequent confirmatory experiments, the Cre-dependent viral tracer, AAVrg-CAG-FLEX-tdTomato-WPRE10GH, was injected into the trachea of heterozygous CGRP-Cre mice (n = 2). Again, animals were anaesthetized with isoflurane (4% for induction and 1.8–2.5% for maintenance), a midline incision was made in the neck and the muscles overlying the trachea were retracted to expose the trachea. Next using a Hamilton syringe, 1 μl of AAVrg-CAG-FLEX-tdTomato-WPRE10GH together with 2μl of saline was injected into the lumen of the trachea. After 4 weeks, the animals were euthanized and their brains, trachea and vagal ganglia were collected for immunohistochemistry. Alternatively, cholera toxin B (CTB, catalogue number #104, List Biological Labs, 0.3% w/v, 5 μl per injection), a retrograde tracer, was injected into the trachea of Phox2b-Cre;loxP-ChR2 mice (n = 6) following the experimental procedures described above. After one week, the animals were euthanized and their brains and vagal ganglia were collected for immunohistochemistry.

    Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Species-specific NLRP3 regulation and its role in CNS autoinflammatory diseases

Koller, B;Nguyen, M;Snouwaert, J;Gabel, C;Ting, J;

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

  • Anthrax lethal toxin List Biological Laboratories Inc. #171B/#172B

    Treatments

    See Table S1 for list of Figures/panels in each figure and corresponding dose and exposure time. General information for the methods is provided here. To induce cytokine release from macrophages, microglia and BMDM cells were primed for 4–5 h (or time indicated in Table S1)with 1 μg/mL of LPS in culture medium, and some cells were pre-treated with 1 μM of CP456773/MCC950 for 30 min before ATP stimulation. Cells were then pulsed with 5 mM ATP for 30 min. For cytokine induction by anthrax lethal toxin (LeTx), LPS-primed cells were treated with LeTx at a final concentration of 250 ng/mL (LF and PA) for 3 h. Cell supernatants were collected and frozen at −80°C until assayed.

    Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
    Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis

HERC5-catalyzed ISGylation potentiates cGAS-mediated innate immunity

Chu, L;Qian, L;Chen, Y;Duan, S;Ding, M;Sun, W;Meng, W;Zhu, J;Wang, Q;Hao, H;Wang, C;Cui, S;

Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

The astrocyte-produced growth factor HB-EGF limits autoimmune CNS pathology

Linnerbauer, M;Lößlein, L;Vandrey, O;Peter, A;Han, Y;Tsaktanis, T;Wogram, E;Needhamsen, M;Kular, L;Nagel, L;Zissler, J;Andert, M;Meszaros, L;Hanspach, J;Zuber, F;Naumann, UJ;Diebold, M;Wheeler, MA;Beyer, T;Nirschl, L;Cirac, A;Laun, FB;Günther, C;Winkler, J;Bäuerle, T;Jagodic, M;Hemmer, B;Prinz, M;Quintana, FJ;Rothhammer, V;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • EAE

    EAE was induced as previously described52. In brief, 8–12-week-old female C57Bl/6J mice were immunized using 150 μg of MOG35–55 (Genemed Synthesis, 110582) mixed with freshly prepared Complete Freund’s Adjuvant (using 20 ml of Incomplete Freund’s Adjuvant (BD Biosciences, no. BD263910) mixed with 100 mg of Myobacterium tuberculosis H37Ra (BD Biosciences, no. 231141)) at a ratio of 1:1 (v/v at a concentration of 5 mg ml−1). All mice received two subcutaneous injections of 100 μl each of the MOG35-55/CFA mix. All mice then received a single intraperitoneal injection of 200 ng of pertussis toxin (List Biological Laboratories, no. 180) at a concentration in 200 μl of PBS. …

    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

A parabrachial hub for the prioritization of survival behavior

Goldstein, N;Maes, A;Allen, H;Nelson, T;Kruger, K;Kindel, M;Smith, N;Carty, J;Villari, R;Cho, E;Marble, E;Khanna, R;Taylor, B;Kennedy, A;Betley, J;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

  • Drugs and Pharmacology

    Formalin (2%, Sigma), PEG (30%, average M.W. 1500, Acros Organics), CFA (50%, Sigma), LiCl (12.5 mg/mL, Sigma), CNO (0.1 mg/mL, Tocris) and Diphtheria toxin (DT, 0.5mg/ml, List Labs) were diluted in normal saline. … DT was injected intramuscularly at a volume of 5 µL/g body weight. Mice received 3 DT injections under light isoflurane anesthesia with 48 h between injections.

    Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

The Dorsal Column Nuclei Scales Mechanical Allodynia During Neuropathic Pain

Upadhyay, A;Gradwell, M;Vajtay, T;Conner, J;Sayal, A;Azadegan, C;Patel, K;Thackary, J;Bohic, M;Imai, F;Ogundare, S;Yoshida, Y;Abdus-Saboor, I;Azim, E;Abraira, V;

Product: Anti-Cholera Toxin B Subunit (Goat)

  • Goat anti-CTb (1:5000, IHC) List Biological Labs 703

    CTb injections

    Hindpaw: Mice were anesthetized (5% induction, 2-3% maintenance). Injections of 1% unconjugated CTb
    (~7µL across four sites) were injected into the ventral paw of C57BL/6 wild type mice to label paw sensory
    input. Animals were perfused 5 days after CTb injection and tissue was processed for IHC as described above.
    Free floating sections were immunostained with goat-CTb (list-labs). 5x images of CTb-labeled afferent inputs
    were taken at the Zeiss LSM 800 confocal microscope and ImageJ was used for synaptic analysis.

    Product #703 – Anti-Cholera Toxin B Subunit (Goat)

RORα is required for expansion and memory maintenance of ILC1s via a lymph node-liver axis

Cheng, M;Li, J;Song, J;Song, H;Chen, Y;Tang, H;Wei, H;Sun, R;Tian, Z;Wang, X;Peng, H;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Bicarbonate signalling via G protein-coupled receptor regulates ischaemia-reperfusion injury

Jo-Watanabe, A;Inaba, T;Osada, T;Hashimoto, R;Nishizawa, T;Okuno, T;Ihara, S;Touhara, K;Hattori, N;Oh-Hora, M;Nureki, O;Yokomizo, T;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

The O-glycan is essential for the induction of protective antibodies against lethal infection by flagella A-bearing Pseudomonas aeruginosa

Choi, M;Shridhar, S;Fox, H;Luo, K;Amin, MN;Tennant, SM;Simon, R;Cross, AS;

Product: LPS from Escherichia coli O111:B4

  • Toll-like receptor (TLR) 4 and 5 activity assays

    TLR4 and TLR5 activity assays were performed as described previously with minor modifications (14, 15). Briefly, HEK-Blue-hTLR4 cells and HEK-Blue-hTLR5 cells carrying a secreted embryonic alkaline phosphatase (SEAP) reporter construct were obtained from InvivoGen (CA). Cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 0.5% penicillin/streptomycin with and without 0.2% Normocin (InvivoGen, CA) for TLR5 cells and TLR4 cells at 37°C with 5% CO2, respectively. Monolayers of 1 × 105 cells per well in a 96-well plate were incubated with media alone, PBS, and FlaA and FlaB proteins at different concentrations ranging from 10 pg/mL to 10 µg/mL for 24 h. E. coli lipopolysaccharide O111:B4 (List Biological Laboratories, Inc., CA) was added to HEK-Blue-hTLR4 cell as a positive control. …

    Product #201 – LPS from Escherichia coli O111:B4

Immunogenicity at delivery after Tdap vaccination in successive pregnancies

De Weerdt, L;Thiriard, A;Leuridan, E;Marchant, A;Maertens, K;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • Tdap-specific IgG and IgG subclass detection

    All available leftover maternal and cord serum samples from both deliveries were tested at the Université Libre de Bruxelles (ULB). Total and subclass Tdap-specific IgG antibodies were quantified using an in-house bead-based multiplex immunoassay (28). In brief, serum samples were incubated with distinct fluorescent magnetic beads, individually coated with specific purified target antigens: PT (List Labs, #180), FHA (Sigma, #F5551), PRN (NAC, #NAT41666), diphtheria toxin (DT, List Labs, #151) and tetanus toxin (TT, MassBiologics, # LP1105P). Samples were measured at appropriate dilutions with a Multigam reference (CAF-DCF, Belgium), a WHO international standard NIBSC 06/140 and blanks included on each plate. Serum dilutions used to titrate IgG and IgG1 were 1:9000-1:18000-1:36000, 1:70-1:140-1:280 for IgG2 and IgG3, and 1:70-1:700-1:7000 for IgG4. After washing, the captured IgG was detected with R-phycoerythrin labelled antibodies specific for each isotype (IgG1-4). …

    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
    Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae