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Page 618 out of 639

Mucosal immune responses in W/Wv and Sl/Sld mutant mice

Yoshino, N;Kanno, H;Takahashi, K;Endo, M;Sato, S;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

... 0.5 µg of CT (List Biological Laboratories, Campbell, Ca, Usa), with a 5-min rest between each inoculation. For intragastric immunization, mice were deprived of ...

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

A protective epitope in type III effector YopE is a major CD8 T cell antigen during primary infection with Yersinia pseudotuberculosis

Zhang, Y;Mena, P;Romanov, G;Lin, JS;Smiley, ST;Bliska, JB;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Mice and immunizations with the YopE69-77 peptide:

Female C57BL/6J mice (Jackson Laboratory) were used for all experiments. Animals that were 9 to 10 weeks old were anesthetized with a mixture of ketamine (60 mg/kg of body weight) and xylazine (10 mg/kg) delivered by intraperitoneal (i.p.) injection. A mixture of 1 μg of cholera toxin (CT; List Biological Laboratories Inc., Campbell, CA) ... Control mice received CT in 15 μl of PBS only. ...

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

PubMed ID: 22064714

Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury

Kim, S;Steelman, AJ;Zhang, Y;Kinney, HC;Li, J;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Mouse models of demyelination:

Age-matched C57BL/6 mice were used in two distinct mouse models of demyelination. ... Immediately following CFA injections, and 24 hours later, mice were injected intraperitoneally with 200 ng of inactivated pertussis toxin (List Biologicals, Campbell, CA). One week after induction of EAE mice received a booster of MOG peptide in Incomplete Freunds Adjuvant (IFA) and control mice were injected with IFA without the MOG peptide. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 21615590

Anthrax vaccine antigen-adjuvant formulations completely protect New Zealand white rabbits against challenge with Bacillus anthracis Ames strain spores

Peachman, KK;Li, Q;Matyas, GR;Shivachandra, SB;Lovchik, J;Lyons, RC;Alving, CR;Rao, VB;Rao, M;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

PA-specific IgG ELISA:

Sera from individual rabbits were assayed in triplicate for the presence of PA-specific IgG by ELISA. Briefly, 96-well flat-bottomed Nunc Maxisorp plates (VWR International, Inc.) were coated with 0.1 μg/well of PA (List Biological Laboratories, Inc.) overnight, blocked with 0.5% casein in phosphate-buffered saline (PBS) for 2 h at 37°C, washed, and incubated for 1 h at 37°C with the test serum. ...

PubMed ID: 22089245

Arthritogenic self-reactive CD4+ T cells acquire an FR4hiCD73hi anergic state in the presence of Foxp3+ regulatory T cells

Martinez, RJ;Zhang, N;Thomas, SR;Nandiwada, SL;Jenkins, MK;Binstadt, BA;Mueller, DL;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Regulatory T cell transfer and Foxp3+ T cell ablation:

Spleens and lymph nodes from either WT or Foxp3DTR B6 3 B6.g7 mice were harvested, and polyclonal CD4+ T cells were purified by negative selection using the CD4+ T cell isolation kit and an LS column (Miltenyi Biotec). To deplete Foxp3-expressing cells from reconstituting polyclonal Foxp3DTR CD4+ T cell populations, DT (List Biological, Campbell, CA) was injected i.p. at a concentration of 50 mg/kg body weight on days 8, 10, and 12 after CD4+ T cell reconstitution (19). ...

PubMed ID: 22124124