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Page 618 out of 659

Identification of immunodominant T cell epitopes from enterotoxigenic E. coli colonization factor antigen I (CFA/I) responsible for T helper cell cytokines

Holderness, K;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Experimental Autoimmune  Encephalomyelitis (EAE) Induction:
 
MOG-dependent EAE was induced in 6- to 8-week old female C57BL/6 mice via
a single peptide dose.  MOG35-55 (Bio-Synthesis, Inc. Lewisville, TX) peptide was
emulsified in complete Freund’s adjuvant (CFA) with 4 mg/mL of killed Mycobacterium
tuberculosis, which was subcutaneously (s.c.) injected in 100 µL into the flank of
anesthetized mice on Day 0.  Mice also received 200 ng of pertussis toxin (PT; List
Biological Laboratories, Inc.
, Campbell, CA) intraperitoneally (i.p.) on Day 0 and Day 2. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

Increased Th17 and regulatory T cell responses in EBV-induced gene 3-deficient mice lead to marginally enhanced development of autoimmune encephalomyelitis

Liu, JQ;Liu, Z;Zhang, X;Shi, Y;Talebian, F;Carl, JW;Yu, C;Shi, FD;Whitacre, CC;Trgovcich, J;Bai, XF;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction and assessment of EAE:

MOG peptide 35–55 (MEVGWYRSPFSRVVHLYRNGK), purchased from Genemed Synthesis (San Antonio, TX), was used as the immunogen. Mice of 8–12 wk of age were immunized s.c. with 200 mg MOG peptide in CFA (containing 400 mg Mycobacterium tuberculosis) in a total volume of 100 ml. Mice also received 150 ng pertussis toxin (List Biological, Campbell, CA) in 200 ml PBS via the tail vein immediately after the immunization and again 48 h later. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22387555

Inflammasome-derived IL-1β regulates the production of GM-CSF by CD4(+) T cells and γδ T cells

Lukens, JR;Barr, MJ;Chaplin, DD;Chi, H;Kanneganti, TD;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE model:

Age- (7–12 wk of age) and sex-matched mice were immunized s.c. with 100 mg myelin oligodendrocyte glycoprotein (MOG)35–55 peptide (MEVGWYRSPFSRVVHLYRNGK) emulsified in CFA (Difco Laboratories) with 500 mg Mycobacterium tuberculosis on day 0. Mice also received 200 ng pertussis toxin (List Biological Laboratories) by i.p. injection on days 0 and 2. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22345669

Monoclonal antibodies conjugated with superparamagnetic iron oxide particles allow magnetic resonance imaging detection of lymphocytes in the mouse brain

Luchetti, A;Milani, D;Ruffini, F;Galli, R;Falini, A;Quattrini, A;Scotti, G;Comi, G;Martino, G;Furlan, R;Politi, LS;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

SJL Mice Immunization:

... At day 7, immunization boost was performed by delivering another identical subcutaneous injection of emulsion on the second shoulder. Mice were also injected intraperitoneally with 100 mL of a pertussis toxin solution (500 ng/100 mL; List Biological Laboratories, Campbell, CA) at days 0, 1, 7, and 8.

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22469239

Neuropeptide Y type 1 receptors are expressed in pre-autonomic neurons in the hypothalamic paraventricular nucleus

Clute-Reinig, NM;Cassaglia, PA;Brooks, VL;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

... To test this hypothesis, we used immunofluorescence to determine if Y1R are present in PVN neurons that project to RVLM, identified by prior RVLM microinjection of the retrograde tracer cholera toxin subunit B (CTb, 0.25%; List Laboratories). ...

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 μl of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA, 0.003 M NaN3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.