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Page 617 out of 644

Cholera toxin activates nonconventional adjuvant pathways that induce protective CD8 T-cell responses after epicutaneous vaccination

Olvera-Gomez, I;Hamilton, SE;Xiao, Z;Guimaraes, CP;Ploegh, HL;Hogquist, KA;Wang, L;Jameson, SC;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Epicutaneous Immunization:

Immunization was typically on the ear skin, without prior treatment. In some experiments (specified in the text) the flank skin was used, in which case mice were shaved (under anesthetic) at least 2 d before immunization (16). ... Unless otherwise indicated, the following doses of adjuvants were used: CT (List Biological Laboratories), 100 μg; CpG 1826 (Invivogen) 500 μg; Poly I:C (Amersham) 200 μg; LPS (Sigma) 10 μg; and imiquimod (3M) 50 μL of 1% cream. The immunization site was extensively washed with water and allowed to dry before the animals recovered. In the case of recombinant CT and CT(mut) used for the OVA–CT fusion studies, 10 μg of CT [or CT(mut)] was used. For sortase fused OVA–CT/CT(mut), the immunization involved a single application step. In titration experiments we found similar adjuvant effects using either 10 or 100 μg of commercial CT for EPI on ear skin, but for experimental consistency data with 100 μg CT are shown throughout. The control mice received PBS in place of antigen or adjuvants, as indicated.

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

PubMed ID: 22308317

A channelopathy contributes to cerebellar dysfunction in a model of multiple sclerosis

Shields, SD;Cheng, X;Gasser, A;Saab, CY;Tyrrell, L;Eastman, EM;Iwata, M;Zwinger, PJ;Black, JA;Dib-Hajj, SD;Waxman, SG;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE:

... The mice also received an injection of 500ng pertussis toxin (List Biological Labs, Campbell, CA) in 200μl 0.1M phosphate-buffered saline intraperitoneally immediately after the first immunization with MOG and again 48 hours later. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22367990

Acamprosate modulates experimental autoimmune encephalomyelitis

Sternberg, Z;Cesario, A;Rittenhouse-Olson, K;Sobel, RA;Leung, YK;Pankewycz, O;Zhu, B;Whitcomb, T;Sternberg, DS;Munschauer, FE;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Reagents:

... Pertussis toxin was obtained from List Biological Laboratories (Campbell, CA, USA). ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 22090150

Assessment of lesion pathology in a new animal model of MS by multiparametric MRI and DTI

Boretius, S;Escher, A;Dallenga, T;Wrzos, C;Tammer, R;Brück, W;Nessler, S;Frahm, J;Stadelmann, C;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

... Pertussis toxin (PTX, List Biological Laboratories, London, UK) was injected on days 0, 2, and 9 after immunization (300 ng ip). Five mice served as controls and were maintained on a normal diet for the duration of the experiment ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 21914485

Birth control vaccine targeting leukemia inhibitory factor

Lemons, AR;Naz, RK;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

... Peptides were also conjugated to cholera toxin B subunit (CTB) (List Biological Labs, Campbell, CA) (peptide:CTB ratio, wt/wt; 2:1) using a two step gluteraldehyde (Sigma-Aldrich) reaction, as described previously (McKenzie and Halsey, 1984; Naz and Chauhan, 2002). ...

Vaccination:

... For i.m./s.c. immunizations, each animal received 50 μl of total volume of the vaccine containing 50 μg of each peptide conjugated to a carrier protein, emulsified with Freund’s adjuvant (Sigma-Aldrich). The first injection was given with Freund’s complete adjuvant, while the subsequent booster injections were given with Freund’s incomplete adjuvant. For i.n. administration, each animal received 10-15 μl volume of the vaccine per nostril containing a total of 50μg of each peptide conjugated to CTB. Each animal received a total of 3-4 booster injections. ...

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 μl of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA, 0.003 M NaN3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

PubMed ID: 22139866