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Page 5 out of 639

Protective effects of PNU‑282987 on sepsis‑induced acute lung injury in mice

Shao, Z;Li, Q;Wang, S;Chen, Z;
Product: LPS from Escherichia coli O55:B5

... agents were added 60 min before the challenge with lipopolysaccharides (LPS) (10 ng/ml, Escherichia coli 055:B5; List Biological Laboratories ...

PubMed ID: 30864715

Valence-encoding in the lateral habenula arises from the entopeduncular region

Li, H;Pullmann, D;Jhou, TC;
Product: Anti-Cholera Toxin B Subunit (Goat)
- Free-floating sections were immunostained for CTb, NeuN or cFos by overnight incubation in 365 goat anti-CTb (List Biological Laboratories, 7032A9, 1: 50,000 dilution), mouse anti-NeuN (Millipore, 366 MAB-377, 1: 5,000 dilution), or rabbit anti-cFos (Millipore, ABE457
PubMed ID: 30855228

The Paraventricular Hypothalamus Regulates Satiety and Prevents Obesity via Two Genetically Distinct Circuits

Li, MM;Madara, JC;Steger, JS;Krashes, MJ;Balthasar, N;Campbell, JN;Resch, JM;Conley, NJ;Garfield, AS;Lowell, BB;
Product: Anti-Cholera Toxin B Subunit (Goat)
Goat anti-Cholera Toxin B List Biological List Biological Cat# 703; RRID: AB_10013220
PubMed ID: 30879785

Axonal damage in spinal cord is associated with gray matter atrophy in sensorimotor cortex in experimental autoimmune encephalomyelitis

Meyer, CE;Gao, JL;Cheng, JY;Oberoi, MR;Johnsonbaugh, H;Lepore, S;Kurth, F;Thurston, MJ;Itoh, N;Patel, KR;Voskuhl, RR;MacKenzie-Graham, A;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Pertussis toxin (500ng; List Biological Laboratories, Campbell, CA) was injected intraperitoneally on days 0 and 2.12
PubMed ID: 30843756

Bacillus anthracis Edema Toxin Inhibits Efferocytosis in Human Macrophages and Alters Efferocytic Receptor Signaling

Pan, Z;Dumas, EK;Lawrence, C;Pate, L;Longobardi, S;Wang, X;James, JA;Kovats, S;Farris, AD;
Product: Anthrax Edema Factor (EF), Recombinant from B. anthracis

Efferocytosis Assay:

Efferocytosis was assessed by flow cytometry [68]. Briefly, 5 × 105/well monocyte-derived human M2(IL-4+IL-10+Dex) macrophages or M2(Dex) macrophages were preincubated with 10 nM recombinant PA (List Biological Laboratories, Campbell, CA, USA), 10 nM recombinant EF (List Biological Laboratories), or various concentrations of PA + EF (ET) for 4 h at 37 °C in 5% CO2 atmosphere. Fluorescently-labeled apoptotic human PMN were preincubated with 10% serum, Protein S, or MFGE8 for 1 h unless otherwise noted and then added to the macrophages, resulting in a macrophage:PMN ratio of 1:5. After 1 h at 37 °C in 5% CO2 atmosphere, the percentage macrophages containing intracellular (eFluor 670+) but not surface-bound (surface CD66b) PMN was assessed by flow cytometry.

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A (Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

PubMed ID: 30866434