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EAE-induced upregulation of mitochondrial MnSOD is associated with increases of mitochondrial SGK1 and Tom20 protein in the mouse kidney cortex

Hira, S;Packialakshmi, B;Zhou, X;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
- injected with PBS (control) or 100 μg of MOG 35-55 (New England Peptide) emulsified in 100 μl complete Freund's adjuvant containing 300 μg Mycobacterium tuberculosis (Fisher Scientific), and with 200 ng pertussis toxin (List Biological Laboratories), intraperitoneally
PubMed ID: 31177508

Epigallocatechin-3-Gallate Prevents Acute Gout by Suppressing NLRP3 Inflammasome Activation and Mitochondrial DNA Synthesis

Lee, HE;Yang, G;Park, YB;Kang, HC;Cho, YY;Lee, HS;Lee, JY;
- St. Louis, MO, USA) and the stock solution was prepared in DMSO. Purified LPS from Escherichia coli was obtained from List Biological Laboratory Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water. Monosodium Cached
PubMed ID: 31174271

Silencing SOCS1 via Liposome-Packed siRNA Sustains TLR4-Ligand Adjuvant

Hildebrand, D;Metz-Zumaran, C;Jaschkowitz, G;Heeg, K;
Product: Tetanus Toxin Light Chain, Recombinant

siRNA/TeTxLC—Liposomes:

Lipid solution (A) of COATSOME® SS-20/3A-P04 (NOF America corporation, White Plains, NY): cholesterol (Merck, Darmstadt, Germany): SUNBRIGHT GM-020 (NOF America corporation, White Plains, NY) = 70:30:3 (mol) was prepared in 90% t-BuOH. siRNA solution (B) was prepared by mixing 100 μl of human SOCS1 siRNA (NM_003745) or contr. siRNA (SIC003) (Sigma-Aldrich, Taufkirchen, Germany) aqueous solution (133 μg/100 μl) and 50 μl 20 mM malate buffer (pH 4.00, 30 mM NaCl). For enclosure of tetanus light chain toxin (List Biological Laboratories, Inc., California, USA) 5 μg/100 μl was added instead of siRNA. While vortexing 400 μl of the lipid solution, siRNA solution (or TeTxLC) was gradually added dropwise and the mixed solution was taken in a 1 ml syringe (C). Then, 225 μl of (C) was injected into 1 ml malate buffer, with vigorous stirring (D). Thereafter, the mixture was further stepwise dialysed with PBS. Adding 2 ml PBS was followed by centrifugation for 1 h at 1,400 rpm in a concentrator plus (Eppendorf, Wiesloch, Germany). Procedure was repeated four times. Final siRNA concentration 66.5 μg/ml. In the experiments 50 μl/ml and 100 μl/ml L-siRNA, 40 μl/ml L- TeTxLC was used.

 

Product #650A, Tetanus Toxin Light Chain, Recombinant, has been discontinued.  Large quantities of Product #650A may be obtained through Bulk Requests and Customer Orders.

PubMed ID: 31214204

Sulfated glycosaminoglycans and low-density lipoprotein receptor contribute to Clostridium difficile toxin A entry into cells

Tao, L;Tian, S;Zhang, J;Liu, Z;Robinson-McCarthy, L;Miyashita, SI;Breault, DT;Gerhard, R;Oottamasathien, S;Whelan, SPJ;Dong, M;
Product: Anti-Clostridium difficile Toxin A (Chicken lgY)
HeLa (H1, CRL-1958), HT-29 (HTB-38), CHO-C6 and 293T (CRL-3216) cells were originally obtained from ATCC. Tey tested negative for mycoplasma contamination, but have not been authenticated. Huh7 and Huh7 LDLR−/− cells were provided by Y. Matsuura (Osaka University)36. Te following mouse monoclonal antibodies were purchased from the indicated vendors: RAC1 (23A8, Abcam), non-glucosylated RAC1 (clone 102, BD Biosciences), β-actin (AC-15, Sigma) and heparan sulfate (F58-10E4, mouse IgM, Amsbio). Rabbit monoclonal IgG against LDLR (EP1553Y) was purchased from Abcam. Chicken polyclonal IgY (753A) against TcdA was purchased from List Biological Labs. Statistical analysis was performed using OriginPro 8 (v.8.0724, OriginLab) sofware.
PubMed ID: 31160825

An unusual and vital protein with guanylate cyclase and P4-ATPase domains in a pathogenic protist

Günay-Esiyok, Ö;Scheib, U;Noll, M;Gupta, N;
Product: Alpha Toxin from C. septicum, Liquid
... rgent and BSA). To test the membrane location of TgATPaseP-GC, the IMC was separated from the plasma membrane by treating extracellular parasites with α-toxin from Clostridium septicum (20 nM, 2 h) (List Biological Laboratories), followed by fixation on BSA-coated coverslips and antibody staining. In both cases, the standard immunostaining procedure was performed subsequently. Lytic cycle assays ... rgent and BSA). To test the membrane location of TgATPaseP-GC, the IMC was separated from the plasma membrane by treating extracellular parasites with α-toxin from Clostridium septicum (20 nM, 2 h) (List Biological Laboratories), followed by fixation on BSA-coated coverslips and antibody staining. In both cases, the standard immunostaining procedure was performed subsequently. Lytic cycle assays
PubMed ID: 31235476