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Page 5 out of 671

The X-linked histone demethylase Kdm6a in CD4+ T lymphocytes modulates autoimmunity

Itoh, Y;Golden, LC;Itoh, N;Matsukawa, MA;Ren, E;Tse, V;Arnold, AP;Voskuhl, RR;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
A booster immunization was applied subcutaneously on day 7 over contralateral lymph nodes. Pertussis toxin (500 ng/mouse) (List Biological Laboratories) was injected intraperitoneally on days 0 and 2 (54).
PubMed ID: 31403472

PTPσ inhibitory peptide promotes recovery of diaphragm function and sprouting of bulbospinal respiratory axons following cervical spinal cord injury

Urban, MW;Ghosh, B;Block, CG;Charsar, BA;Smith, GM;Wright, MC;Li, S;Lepore, AC;
Product: Anti-Cholera Toxin B Subunit (Goat)

... anti‐CTB at 1:10,000 (List Biological Laboratories, Campbell, CA). ...

PubMed ID: 31392919

Glutamatergic Input Varies with Phrenic Motor Neuron Size

Rana, S;Mantilla, CB;Sieck, GC;
Product: Anti-Cholera Toxin B Subunit (Goat)

... An anti-CTB (Goat polyclonal, Cat #703, Lot # 7032A9, 1:5000; List Biologicals) was used to label PhMNs. ...

PubMed ID: 31389739

DPP8/9 inhibitors are universal activators of functional NLRP1 alleles

Gai, K;Okondo, MC;Rao, SD;Chui, AJ;Ball, DP;Johnson, DC;Bachovchin, DA;
Product: Anthrax Lethal Factor (LF),Recombinant from B. anthracis

Reagents and antibodies

... For cell culture experiments, Val-boroPro was resuspended in DMSO containing 0.1% TFA to prevent compound cyclization. Anthrax Lethal Factor (Catalog #172B) and PA (Catalog #171E) were purchased from List Labs, ...

LDH cytotoxicity assays

For LDH release experiments involving primary macrophages, mBMDM and rBMDM were isolated from the femurs and tibias of 7–9-week-old male and female mice and 7–12-week-old male rats. 1 × 107 cells were plated on 10 cm Petri dishes. mBMDMs were differentiated in DMEM with 10% FBS and 20% L Cell medium for 6 days and rBMDMs in 30% L Cell medium for 7–9 days. Macrophages were scraped and reseeded at 0.25 × 106 cells/well in 12-well plates in Opti-MEM and incubated overnight before treatment. Cells were then treated as indicated with compounds or LT. ...

In vitro and in cell LF cleavage assays

HEK 293T cells were seeded at 0.5 × 106 cells/well in six-well plates and transiently transfected with 2 µg of the indicated plasmids using FuGENE HD. For the in vitro assay, lysates were harvested after 48 h and normalized to 1 mg/mL using the DC Protein Assay kit (Bio-Rad). These lysates were then incubated with LF (1 µg/mL) for the indicated time periods at 25 °C quenched by boiling with 2 × SDS–PAGE sample buffer at the indicated times, separated by SDS–PAGE, immunoblotted, and visualized using the Odyssey Imaging System (Li-Cor). For the in cell assay, after 48 h after transfection cells were treated with LT (1 µg/mL, 6 h) before lysates were harvested for immunoblotting as described above.

PubMed ID: 31383852

Salivary gland macrophages assist tissue-resident CD8+ T cell immune surveillance

Stolp, B;Thelen, F;Ficht, X;Altenburger, L;Ruef, N;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Antibodies and reagents:

...Pertussis toxin (PTx) and enzymatically inactive mutant PTx (PTxmut) were obtained from List Biological Laboratories

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).