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Page 4 out of 639

Innervated Properties of Acupuncture Points LI 4 and LR 3 in the Rat: Neural Pathway Tracing with Cholera Toxin Subunit B

Cui, J;Wang, J;Bai, W;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

...Under anesthesia with isoflurane, a total of 4 μL of 1% CTB (List Biological Labs, Campbell, CA) solution was injected slowly into LI 4 or LR 3 on the left side. The depth of the injection was ∼2-3 mm. FIG. 1. LI 4 (A) and LR 3 (B) in rat forefoot and hind foot respectively...

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 μl of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA, 0.003 M NaN3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

A comprehensive map of the dendritic cell transcriptional network engaged upon innate sensing of HIV

Johnson, JS;De Veaux, N;Rives, AW;Lahaye, X;

Infections and stimulations:

DCs were infected or stimulated with innate agonists between day 4 and day 6 after differentiation. DCs were counted on day 4 and resuspended at 800,000 cell per ml in fresh medium with GM-CSF, IL-4, and polybrene (1 µg/ml) and then reseeded into appropriate culture vessels. For most assays, DCs were infected at a density of 800,000 cells per ml by diluting virus in DC media (without cytokines or polybrene) to a final volume normalized to controls. Innate and inflammatory stimuli were used at the indicated concentrations: polyI:C (InvivoGen, 10 µg/ml); LPS (List Biological Laboratories, INC, 1 ng/ml).

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://www.listlabs.com/product-information/lipopolysaccharides/

Testing the resource trade-off hypothesis for carotenoid-based signal honesty using genetic variants of the domestic canary

Koch, RE;Staley, M;Kavazis, AN;Hasselquist, D;Toomey, MB;Hill, GE;
Product: LPS from Escherichia coli O55:B5

Briefly, we isolated a subset of birds in experimental cages the day before LPS injection, then dosed them the following morning with an intra-abdominal injection of 1 mg ml−1 LPS from E. coli (O55:B5; List Biological Laboratories, Campbell, CA, USA) dissolved in PBS. ...

PubMed ID: 30877227

Alpinia oxyphylla Fruit Extract Ameliorates Experimental Autoimmune Encephalomyelitis through the Regulation of Th1/Th17 Cells

Huang, K;Lin, M;Hsu, Y;Lu, I;Pan, I;Yang, J;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Chemicals and Reagents:

... Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA, USA) ...

EAE in Mice:

Female C57BL/6 mice at 10–12 weeks of age were immunized with subcutaneous injection of 200-g MOG35-55 peptide and 400-g mycobacterium tuberculosis H37Ra in a 200-L emulsion. Te mice received intraperitoneal injection of 500-ng pertussis toxin on Day 0 and Day 2. On Day 7 afer immunization or the onset of EAE symptoms, the mice were divided into groups and treated for 14 days. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

Central expression of synaptophysin and synaptoporin in nociceptive afferent subtypes in the dorsal horn

Chung, J;Franklin, JF;Lee, HJ;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
... d DCNs at T7 and T13 spinal levels were exposed through a skin incision along the median line across thoracic areas. Transganglionic axonal tracers, IB4 (Vector Laboratories, Burlingame, CA) and CTB (List Biological Laboratories, Campbell, CA) were dissolved in sterile phosphate buffered saline (PBS; pH 7.4) for injections (1 ul of 2% solution for each tracer). IB4 was injected into left DCNs and ... d DCNs at T7 and T13 spinal levels were exposed through a skin incision along the median line across thoracic areas. Transganglionic axonal tracers, IB4 (Vector Laboratories, Burlingame, CA) and CTB (List Biological Laboratories, Campbell, CA) were dissolved in sterile phosphate buffered saline (PBS; pH 7.4) for injections (1 ul of 2% solution for each tracer). IB4 was injected into left DCNs and
PubMed ID: 30862809