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Page 4 out of 671

MicroRNA 182 promotes T helper 1 cell by Repressing Hypoxia Induced Factor 1 alpha in experimental autoimmune encephalomyelitis

Wan, C;Bi, W;Lin, P;Zhang, Y;Tian, J;Fang, S;Li, Z;Wang, X;Qu, Y;Mu, L;Wang, J;Kong, Q;Wang, G;Sun, B;Li, H;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

... 50μl of the emulsion were injected in each axillary fossa. 200ng of pertussis toxin (LIST BIOLOGICAL LABORATORIES, INC) were administered intravenously at the time of immunization and 48 h later. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 31411745

BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling

Choi, S;Yu, J;Park, A;Dubon, MJ;Do, J;Kim, Y;Nam, D;Noh, J;Park, KS;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture:

MCF-10A cells were obtained from ATCC (Manassas, VA, USA) and were grown using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12, 1:1; Invitrogen, Carlsbad, CA, USA) supplemented with 5% horse serum (Invitrogen), 20 ng/ml recombinant human epidermal growth factor (EGF; R&D systems, Minneapolis, MN, USA), 0.5 μg/ml hydrocortisone (Sigma, St. Louis, MO, USA), 100 ng/ml cholera toxin (List Biological Lab, St, Campbell, CA, USA), ...

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

PubMed ID: 31409851

Intratelencephalic projections of the avian visual dorsal ventricular ridge: Laminarly segregated, reciprocally and topographically organized

Fernández, M;Ahumada-Galleguillos, P;Sentis, E;Marín, G;Mpodozis, J;
Product: Anti-Cholera Toxin B Subunit (Goat)

- List Biological Laboratories Inc., Campbell, CA, USA; Cat# 703; goat, polyclonal; RRID: AB_10013220 1:80,000 Anti-PHA-L PHA-L (Phaseolus vulgaris leucoagglutinin) Choleragenoid, List Biological Laboratories Inc., Campbell, CA, USA) in distilled water ...

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 μl of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA, 0.003 M NaN3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

PubMed ID: 31407337

Dexras1 Deletion and Iron Chelation Promote Neuroprotection in Experimental Optic Neuritis

Khan, RS;Baumann, B;Dine, K;Song, Y;Dunaief, JL;Kim, SF;Shindler, KS;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction and scoring of EAE:

EAE was induced according to prior studies23,24. Briefly, 8 week old female C57BL/6J mice (N = 18) were anesthetized with isoflurane and were injected subcutaneously at two sites on the back with a total of 200 μg of myelin oligodendrocyte glycoprotein (MOG) peptide (MOG35–55; Genscript, Piscataway, NJ, USA) emulsified in Complete Freund’s Adjuvant (Difco, Detroit, MI, USA) containing 2.5 mg/ml mycobacterium tuberculosis (Difco). Control mice (N = 9) were injected with an equal volume of phosphate buffered saline (PBS) and Complete Freund’s Adjuvant. Each animal also received 200 ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1 ml PBS by intraperitoneal injection at 0 h and 48 h post-immunization. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 31406150

Placental growth factor regulates the generation of TH17 cells to link angiogenesis with autoimmunity

Yoo, SA;Kim, M;Kang, MC;Kong, JS;Kim, KM;Lee, S;Hong, BK;Jeong, GH;Lee, J;Shin, MG;Kim, YG;Apicella, I;Cicatiello, V;De Falco, S;Yoon, CH;Cho, CS;Ryoo, ZY;Lee, SH;Kim, WU;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Wild-type and Plgf-KO mice on the C57BL/6N background were subcutaneously immunized with 200μg of MOG35–55 peptide (MEVGWYRSPFSRVVHLYRNGK) emulsified in 100μl CFA (Sigma-Aldrich) in the abdominal flank. On days 0 and 2, the immunized mice received additional intraperitoneal injections of 200ng of pertussis toxin (List Biological Labs).
PubMed ID: 31406382