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Page 3 out of 639

Zeptomole per milliliter detection and quantification of edema factor in plasma by LC-MS/MS yields insights into toxemia and the progression of inhalation anthrax

Lins, RC;Boyer, AE;Kuklenyik, Z;Woolfitt, AR;Goldstein, J;Hoffmaster, AR;Gallegos-Candela, M;Leysath, CE;Chen, Z;Brumlow, JO;Quinn, CP;Bagarozzi, DA;Leppla, SH;Barr, JR;
Product: Anthrax Protective Antigen, Activated (PA 63) from B. anthracis
- Recombinant EF was produced as previously described [17]. Activated recombinant PA (PA63) was from List Biological Laboratories (Campbell, CA). Pertussis adenylyl cyclase toxin (CyaA) was from Sigma-Aldrich (St. Louis, MO)
PubMed ID: 30911800

Accurate and selective quantification of anthrax protective antigen in plasma by immunocapture and isotope dilution mass spectrometry

Solano, MI;Woolfitt, AR;Boyer, AE;Lins, RC;Isbell, K;Gallegos-Candela, M;Moura, H;Pierce, CL;Barr, JR;
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
- These measurements were compared to previously published PA, LF, PCR and bacteremia results. 24. Experimental procedures. Materials. Recombinant anthrax toxins PA83, PA63, LF and EF were from List Biological Laboratories (Campbell, CA)
PubMed ID: 30810119

Phenotypic Screening-Based Identification of 3,4-Disubstituted Piperidine Derivatives as Macrophage M2 Polarization Modulators: An Opportunity for Treating Multiple Sclerosis

Weng, Q;Che, J;Zhang, Z;Zheng, J;Zhan, W;Lin, S;Tian, T;Wang, J;Gai, R;Hu, Y;Yang, B;He, Q;Dong, X;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Then, the mixture was injected subcutaneously into neck (0.1 mL) and both hindlegs (0.05 mL each) of mice. Pertussis toxin (200 ng; List Biological Lab, Inc) was administered intravenously on days 0 and 1 post-immunization.
PubMed ID: 30856328

Outbreak of Murine Infection with Clostridium difficile Associated with the Administration of a Pre- and Perinatal Methyl Donor Diet

Mau, T;Eckley, SS;Bergin, IL;Saund, K;Villano, JS;Vendrov, KC;Snitkin, ES;Young, VB;Yung, R;
Product: Toxin A from Clostridium difficile

C. difficile cytotoxin assay:

A cytotoxicity assay was performed as previously described with the following modifications (25). Briefly, ATCC CCL-81 Vero cells were grown to confluence in Dulbecco modified Eagle medium (Gibco catalog number 11965) supplemented with 10% fetal bovine serum (catalog number 16140) and 1% penicillin-streptomycin (Gibco catalog number 15070). Cells were plated to a density of 10 5 cells/well. Mouse cecal content was diluted 1:10 in sterile PBS, passed through a 0.22- m filter, and serially diluted to 10:6. Filtered samples were tested in duplicate with a corresponding control to which both antitoxin (Techlab catalog number T5000) and sample were added. A positive control of C. difficile TcdA (List Biologicals catalog number 152C) was used. Samples were incubated overnight at 37°C, and the cytotoxic titer was determined as the reciprocal of the highest dilution that produced 80% cell rounding.

PubMed ID: 30894434

Systemic administration of orexin A ameliorates established experimental autoimmune encephalomyelitis by diminishing neuroinflammation

Becquet, L;Abad, C;Leclercq, M;Miel, C;Jean, L;Riou, G;Couvineau, A;Boyer, O;Tan, YV;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
- mL of Mycobacterium tuberculosis H37Ra (Difco, USA). In addition, mice received intraperitoneally (IP) 300 ng of pertussis toxin (List Laboratories, USA) on days 0 and 2 post-immunization. Mice were scored daily from 0 to Cached
PubMed ID: 30894198