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Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin

Farcasanu, M;Wang, AG;Uchański, T;Bailey, LJ;Yue, J;Chen, Z;Wu, X;Kossiakoff, A;Tang, WJ;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
The column was washed with T20β5N100P0.1 followed by T20β5N100P0.1 plus 20 mM imidazole and eluted with T20β5N100P0.1 with 150 mM imidazole. Peak fractions were pooled and diluted tenfold with T20P0.1, loaded onto a Source Q anion exchange column, and eluted by a 0-1M NaCl gradient. Purified EF was concentrated to approximately 20 mg/mL and frozen at -80°C. Protein quantitation was performed using extinction coefficients calculated from the known primary sequence of each protein on the Expasy ProtParam webserver. PA was purchased from List Labs.
PubMed ID: 31243996

TET1 is an important transcriptional activator of TNFα expression in macrophages

Sun, F;Abreu-Rodriguez, I;Ye, S;Gay, S;Distler, O;Neidhart, M;Karouzakis, E;
- THP-1 cells were differentiated into macrophages in the presence of 50 ng/ml phorbol myristate acetate (PMA, Sigma, USA) for 48 hours. THP-1 PMA-derived macrophages were stimulated with 10 ng/ml E. coli LPS (List Biological Laboratories, California) for 2 hours Cached
PubMed ID: 31216336

The Alzheimer's Disease-Associated Protein BACE1 Modulates T Cell Activation and Th17 Function

Hernandez-Mir, G;Raphael, I;Revu, S;Poholek, CH;Avery, L;Hawse, WF;Kane, LP;McGeachy, MJ;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

... A total of 200 ng of Pertussis Toxin (List Biological Laboratories) was given ip on day 0 and 2. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

PubMed ID: 31209103

Stimulation of alpha 7 nicotinic acetylcholine receptor (α7nAChR) inhibits atherosclerosis via immunomodulatory effects on myeloid cells

Ulleryd, MA;Mjörnstedt, F;Panagaki, D;Yang, LJ;Engevall, K;Gutiérrez, S;Wang, Y;Gan, LM;Nilsson, H;Michaëlsson, E;Johansson, ME;
; - Samples were challenged with 10 ng mL-1 of lipopolysaccharide (LPS, List Biological Laboratories inc.) and treated with PBS or increasing concentrations of AZ6983, 0.1, 1, 10 or 100 μmol L-1. Control samples also included the addition of 100 μmol L-1 of AZ6983

Connections of the laterodorsal tegmental nucleus with the habenular-interpeduncular-raphe system

Bueno, D;Lima, LB;Souza, R;Gonçalves, L;Leite, F;Souza, S;Furigo, IC;Donato, J;Metzger, M;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
- Page 1. RESEARCH ARTICLE Associate Editor: Paul E. Sawchenko Connections of the Laterodorsal Tegmental Nucleus with the Habenular- Interpeduncular- Raphe System Debora Bueno, 1 Leandro B. Lima, 1 Rudieri Souza ; - tracer Phaseolus vulgaris-leucoagglutinin (PHA-L; 2.5% in 0.1 M phosphate buffer, Vector Laboratories, Burlingame, CA,) and the retrograde tracer cholera toxin subunit b (CTb; low salt, 1% in distilled water, List Biological Laboratories Inc., Campell, CA) were used
PubMed ID: 31199515