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Sialomucin CD43 regulates Th17 cell ICAM-1 dependent adhesion, apical migration and transendothelial migration

Velázquez, FE;Anastasiou, M;Carrillo-Salinas, FJ;Ngwenyama, N;Salvador, AM;Nevers, T;Alcaide, P;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
- and Alexa 680 and Phaloidin Alexa Fluor 546 were from Life Technologies (Carlsbad, CA). Myelin oligodendrocyte glycoprotein (MOG) was purchased from Anaspec (Fremont, CA) and Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA
PubMed ID: 30690734

Therapeutic Potential of Conditioned Medium Derived from Oligodendrocytes Cultured in a Self-Assembling Peptide Nanoscaffold in Experimental Autoimmune Encephalomyelitis

Jahanbazi Jahan-Abad, A;Karima, S;Sahab Negah, S;Noorbakhsh, F;Borhani-Haghighi, M;Gorji, A;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
- The mice also received ip injection of pertussis toxin (300 ng in 100 μl PBS; List Biological Lab, USA) at the time of immunization and again 48 hours later.(Majidi-Zolbanin et al., 2015) For dose-response studies, C57BL/6 mice were randomly divided into four groups
PubMed ID: 30703369

Antitumour activity of Helix hemocyanin against bladder carcinoma permanent cell lines

Dolashki, A;Dolashka, P;Stenzl, A;Stevanovic, S;Aicher, W;Velkova, L;Velikova, R;Voelter, W;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
Normal human urothelial cells were grown to confluence in complete keratinocyte serum-free medium (cKSFM) with human recombinant epithelial growth factor (5 ng/mL) bovine, pituitary extract (50 mg/mL; Gibco), supplemented with cholera toxin (30 ng/mL; List Biological Laboratories, Campbell, CA, USA) under standard conditions.

A Novel Supplementation Approach to Enhance Host Response to Sublingual Vaccination

Rowe, JC;Attia, Z;Kim, E;Cormet-Boyaka, E;Boyaka, PN;
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
... lutions were added to J774 macrophages cultured in cultured in RPMI supplemented with 10% fetal calf serum. Bacillus anthracis lethal toxin (LeTx) [i.e., PA plus Bacillus anthracis lethal factor (LF, List Biological, Campbell, CA)] was then added to the plates. After overnight incubation, MTT (3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyl tetrazolium bromide; Sigma-Aldrich) was added to assess the ... lutions were added to J774 macrophages cultured in cultured in RPMI supplemented with 10% fetal calf serum. Bacillus anthracis lethal toxin (LeTx) [i.e., PA plus Bacillus anthracis lethal factor (LF, List Biological, Campbell, CA)] was then added to the plates. After overnight incubation, MTT (3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyl tetrazolium bromide; Sigma-Aldrich) was added to assess the
PubMed ID: 30679470

Neuron-specific PERK inactivation exacerbates neurodegeneration during experimental autoimmune encephalomyelitis

Stone, S;Yue, Y;Stanojlovic, M;Wu, S;Karsenty, G;Lin, W;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
... ptide emulsified in complete Freund’s adjuvant (BD Biosciences) supplemented with 600 μg _Mycobacterium tuberculosis_ (strain H37Ra; BD Biosciences). Two i.p. injections of 400 ng pertussis toxin (List Biological Laboratories) were given 0 and 48 hours later. Clinical scores (0, healthy; 1, flaccid tail; 2, ataxia and/or paresis of hind limbs; 3, paralysis of hind limbs and/or paresis of foreli ... ptide emulsified in complete Freund’s adjuvant (BD Biosciences) supplemented with 600 μg _Mycobacterium tuberculosis_ (strain H37Ra; BD Biosciences). Two i.p. injections of 400 ng pertussis toxin (List Biological Laboratories) were given 0 and 48 hours later. Clinical scores (0, healthy; 1, flaccid tail; 2, ataxia and/or paresis of hind limbs; 3, paralysis of hind limbs and/or paresis of foreli
PubMed ID: 30674717