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Page 2 out of 671

Vitamin D receptor and metabolite effects on corneal epithelial cell gap junction proteins

Lu, X;Chen, Z;Sarah, V;Watsky, MA;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

... the half corneal button securely attached to the bottom of the plate, and 1.5 mL of DMEM (Life technology) with 10% serum containing 40 μg/mL gentamicin (Life technologies, NY), 1% ITS (Fisher Scientific, PA), and 100 ng/mL cholera toxin (LIST Biological Laboratories) ...

 

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C - Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B - Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders

PubMed ID: 31465769

Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver

Choudhury, SR;Babes, L;Rahn, JJ;Ahn, BY;Goring, KR;King, JC;Lau, A;Petri, B;Hao, X;Chojnacki, AK;Thanabalasuriar, A;McAvoy, EF;Tabariès, S;Schraeder, C;Patel, KD;Siegel, PM;Kopciuk, KA;Schriemer, DC;Muruve, DA;Kelly, MM;Yipp, BG;Kubes, P;Robbins, SM;Seng
Product: ULTRA PURE LPS from Escherichia coli O111:B4
Ultrapure LPS (Escherichia coli 111B4) List Biological Laboratories. INC Cat# 421
PubMed ID: 31442408

Fasting-Refeeding Impacts Immune Cell Dynamics and Mucosal Immune Responses

Nagai, M;Noguchi, R;Takahashi, D;Morikawa, T;Koshida, K;Komiyama, S;Ishihara, N;Yamada, T;Kawamura, YI;Muroi, K;Hattori, K;Kobayashi, N;Fujimura, Y;Hirota, M;Matsumoto, R;Aoki, R;Tamura-Nakano, M;Sugiyama, M;Katakai, T;Sato, S;Takubo, K;Dohi, T;Hase, K;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
Cholera toxin (CT) List Biological Laboratories Cat#100B
PubMed ID: 31442401

Inhibition of NLRP3 inflammasome in tumor microenvironment leads to suppression of metastatic potential of cancer cells

Lee, HE;Lee, JY;Yang, G;Kang, HC;Cho, YY;Lee, HS;Lee, JY;

Reagents:

Purified LPS from Escherichia coli was obtained from List Biological Laboratory Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water. ...

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://www.listlabs.com/product-information/lipopolysaccharides/

PubMed ID: 31439870

Antigen-specific Mucosal Immunity Regulates Development of Intestinal Bacteria-mediated Diseases

Fujimoto, K;Kawaguchi, Y;Shimohigoshi, M;Gotoh, Y;Nakano, Y;Usui, Y;Hayashi, T;Kimura, Y;Uematsu, M;Yamamoto, T;Akeda, Y;Rhee, JH;Yuki, Y;Ishii, KJ;Crowe, SE;Ernst, PB;Kiyono, H;Uematsu, S;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
CT (10 µg/ml, List Biological Laboratories) was dissolved alone or in combination with CpG-ODN and curdlan in PBS, emulsified with IFA, and then injected intramuscularly as described above.
PubMed ID: 31445037