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Page 2 out of 659

Antigen-specific CD4+ CD25+ T cells induced by locally expressed ICOS-Ig: The role of Foxp3, Perforin, Granzyme B and IL-10

Christiansen, D;Mouhtouris, E;Hodgson, R;Sutton, VR;Trapani, JA;Ierino, FL;Sandrin, MS;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
- In vivo depletion of Foxp3 + Treg Foxp3 + Treg were depleted from DEREG mice[10] by injection of 500ng Diphtheria toxin (DT) (List Biological Laboratories, Inc., Campbell, CA) and two days later mice were grafted as described below
PubMed ID: 31225919

Bidirectional regulatory potentials of short-chain fatty acids and their G-protein-coupled receptors in autoimmune neuroinflammation

Park, J;Wang, Q;Wu, Q;Mao-Draayer, Y;Kim, CH;
Product: Enterotoxin Type B from Staphylococcus aureus

Experimental autoimmune encephalomyelitis (EAE) in mice:

For induction of EAE, 8–10-week-old female mice were immunized with the MOG35–55 peptide (100 μg/mouse, AnaSpec) in complete Freund’s adjuvant (Difco Laboratories). On day 0 and day 2, pertussis toxin (100 ng/mouse, List Biological Labs) was injected intraperitoneally. EAE was also induced with adoptive transfer of MOG-stimulated T cells. For this, C57BL/6 mice were immunized with MOG35–55 peptide. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).


Cell isolation and culture:

Naïve CD4+ T cells were isolated from splenocytes and lymph node cells with the naive CD4+ T cell isolation kit (Miltenyi Biotec). To obtain CNS APCs, brains from newborn mice were collected, dissociated with scissors and digested with collagenase type 4 (2 mg/ml, Worthington Biochemical) for 1 hour at 37 °C. Mononuclear cells in the interphase between 30% and 70% of Percoll (GE healthcare) were isolated by density-cut centrifugation as described38. These cells were cultured for 4 days and then activated by TNF-α (10 ng/ml, BioLegend) and IL-17 (25 ng/ml, BioLegend) in the presence and absence of SCFAs (C2 at 10 mM, C3 at 1 mM, C4 at 0.5 mM) for additional 3 days in DMEM medium (10% FBS). Adherent glial cells, largely composed of microglial cells, were co-cultured as APCs with naïve CD4+ T cells at 1:10 ratio for 5–6 days in the presence of Staphylococcal enterotoxin B (SEB, 5 μg/ml, List Labs) to stimulate naïve CD4+ T cells isolated from the spleen and lymph nodes of unimmunized C57BL/6 mice.

PubMed ID: 31222050

Distinct Spatiotemporal Distribution of Bacterial Toxin-Produced Cellular cAMP Differentially Inhibits Opsonophagocytic Signaling

Hasan, S;Rahman, WU;Sebo, P;Osicka, R;
Product: Anthrax Edema Factor (EF), Recombinant from B. anthracis
- as determined by Limulus Amebocyte Lysate assay (QCL-1000, Lonza, Walkersville, MD) and was stored in 50 mM Tris (pH 8.0), 8 M urea, and 2 mM CaCl 2 buffer at ‒20 °C. Recombinant EF and PA from Bacillus anthracis were purchased from List Biological Laboratories Cached
PubMed ID: 31226835

HSC70 and HSP90 chaperones perform complementary roles in translocation of the cholera toxin A1 subunit from the endoplasmic reticulum to the cytosol

Burress, H;Kellner, A;Guyette, J;Tatulian, SA;Teter, K;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
- Sigma- Aldrich (St. Louis, MO). DT and CT were purchased from List Biological Laboratories (Campbell, CA). His-tagged CTA1 and 13C- labeled CTA1 were purified in the lab as previously described (49,62). HSC70 was purchased
PubMed ID: 31221799

Pyruvate kinase M2 is requisite for Th1 and Th17 differentiation

Kono, M;Maeda, K;Stocton-Gavanescu, I;Pan, W;Umeda, M;Katsuyama, E;Burbano, C;Orite, SYK;Vukelic, M;Tsokos, MG;Yoshida, N;Tsokos, GC;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Five million cells were suspended in 150 μl of PBS (pH 7.4) and injected intravenously into each Rag1-deficient mouse. Pertussis toxin (300 ng per mouse; List Biological Laboratories) was intraperitoneally injected later on the day of transfer and 2 days later. Mice were monitored and weighed as previously described (11).
PubMed ID: 31217348