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Single-cell transcriptomic analysis of the lateral hypothalamic area reveals molecularly distinct populations of inhibitory and excitatory neurons

Mickelsen, LE;Bolisetty, M;Chimileski, BR;Fujita, A;Beltrami, EJ;Costanzo, JT;Naparstek, JR;Robson, P;Jackson, AC;
Product: Anti-Cholera Toxin B Subunit (Goat)
Following the FISH protocol, sections were blocked for 10 min in 10% donkey normal serum (DNS) in phosphate-buffered saline (PBS) solution supplemented with 0.2% Tween-20 (PBST), then incubated for 1 h with goat anti-CTb (1:2,000; List Biological Laboratories, no. 703, lot no. 7032A10).
PubMed ID: 30858605

Potent Neutralization of Staphylococcal Enterotoxin B in vivo by Antibodies that Block Binding to the T-Cell Receptor

Chen, G;Karauzum, H;Long, H;Carranza, D;Holtsberg, FW;Howell, KA;Abaandou, L;Zhang, B;Jarvik, N;Ye, W;Liao, GC;Gross, ML;Leung, DW;Amarasinghe, GK;Aman, MJ;Sidhu, SS;
Product: LPS from Escherichia coli O55:B5
Lipopolysaccharide (LPS, Escherichia coli 055:B5) was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) and reconstituted with phosphate-buffered saline (PBS) buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, pH 7.4) prior to use.
PubMed ID: 30928493

Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells

Fleskens, V;Minutti, CM;Wu, X;Wei, P;Pals, CEGM;McCrae, J;Hemmers, S;Groenewold, V;Vos, HJ;Rudensky, A;Pan, F;Li, H;Zaiss, DM;Coffer, PJ;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
6 to 8 weeks old, sex-matched NLKfl/fl and NLKΔTreg mice littermates were injected subcutaneously in the rear flank with 100 ug MOG35-55 peptide (2HNMEVGWYRSPFSRVVHLYRNGK-COOH) in complete Freund’s adjuvant (CFA) (Sigma) on day 0, and 250ng pertussis toxin (List Biological) was injected intraperitoneally on day 0 and day 2 post-induction.
PubMed ID: 30917315

Single cell RNA sequencing identifies TGFβ as a key regenerative cue following LPS-induced lung injury

Riemondy, KA;Jansing, NL;Jiang, P;Redente, EF;Gillen, AE;Fu, R;Miller, AJ;Spence, JR;Gerber, AN;Hesselberth, JR;Zemans, RL;
Product: LPS from Escherichia coli O111:B4


Animals were maintained in a pathogen-free environment on a 12-hour light/12-hour dark cycle with full access to food and water. SftpcCreERT2mice (59), obtained from Harold Chapman (UCSF, San Francisco, California, USA), were crossed to Rosa26-mTmG (abbreviated mTmG) mice (The Jackson Laboratory). Male SftpcCreERT2+/–;mTmG+/– mice were given 20 mg/ml tamoxifen in corn oil at a dose of 0.25 mg/g body weight i.p. every other day for 3 doses at age 6 weeks. Recombination efficiency is shown in Supplemental Figure 1. At 12 weeks of age, mice were treated with 40 μg LPS (E. coli 0111:B4, List Biological Laboratories) in 50 μl 0.9% saline by intratracheal instillation or left untreated. Mice were randomly assigned to treatment groups. Mice were euthanized 7 or 27 days later. Sprague-Dawley rats aged 2–4 months were purchased from Charles River or obtained from the University of Michigan Rodent Recycling Program. Tgfbr2fl/fl mice were purchased from The Jackson Laboratory.

PubMed ID: 30913038

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

Tietz, S;Engelhardt, B;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer


... Pertussis toxin e.g. List biological laboratories, Inc. 180 ...