Animals were maintained in a pathogen-free environment on a 12-hour light/12-hour dark cycle with full access to food and water. SftpcCreERT2mice (59), obtained from Harold Chapman (UCSF, San Francisco, California, USA), were crossed to Rosa26-mTmG (abbreviated mTmG) mice (The Jackson Laboratory). Male SftpcCreERT2+/–;mTmG+/– mice were given 20 mg/ml tamoxifen in corn oil at a dose of 0.25 mg/g body weight i.p. every other day for 3 doses at age 6 weeks. Recombination efficiency is shown in Supplemental Figure 1. At 12 weeks of age, mice were treated with 40 μg LPS (E. coli 0111:B4, List Biological Laboratories) in 50 μl 0.9% saline by intratracheal instillation or left untreated. Mice were randomly assigned to treatment groups. Mice were euthanized 7 or 27 days later. Sprague-Dawley rats aged 2–4 months were purchased from Charles River or obtained from the University of Michigan Rodent Recycling Program. Tgfbr2fl/fl mice were purchased from The Jackson Laboratory.
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