Citations

3291 total record number
202 records this year

To narrow your search, use one or more of the following search menus below.

To search by keyword, you may search by type of cell/animal/assay/protein/research or publication.

Page 2 out of 659

Antigen-specific CD4+ CD25+ T cells induced by locally expressed ICOS-Ig: The role of Foxp3, Perforin, Granzyme B and IL-10

Christiansen, D;Mouhtouris, E;Hodgson, R;Sutton, VR;Trapani, JA;Ierino, FL;Sandrin, MS;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
- In vivo depletion of Foxp3 + Treg Foxp3 + Treg were depleted from DEREG mice[10] by injection of 500ng Diphtheria toxin (DT) (List Biological Laboratories, Inc., Campbell, CA) and two days later mice were grafted as described below
PubMed ID: 31225919

Bidirectional regulatory potentials of short-chain fatty acids and their G-protein-coupled receptors in autoimmune neuroinflammation

Park, J;Wang, Q;Wu, Q;Mao-Draayer, Y;Kim, CH;
Product: Enterotoxin Type B from Staphylococcus aureus

Experimental autoimmune encephalomyelitis (EAE) in mice:

For induction of EAE, 8–10-week-old female mice were immunized with the MOG35–55 peptide (100 μg/mouse, AnaSpec) in complete Freund’s adjuvant (Difco Laboratories). On day 0 and day 2, pertussis toxin (100 ng/mouse, List Biological Labs) was injected intraperitoneally. EAE was also induced with adoptive transfer of MOG-stimulated T cells. For this, C57BL/6 mice were immunized with MOG35–55 peptide. ...

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

 

Cell isolation and culture:

Naïve CD4+ T cells were isolated from splenocytes and lymph node cells with the naive CD4+ T cell isolation kit (Miltenyi Biotec). To obtain CNS APCs, brains from newborn mice were collected, dissociated with scissors and digested with collagenase type 4 (2 mg/ml, Worthington Biochemical) for 1 hour at 37 °C. Mononuclear cells in the interphase between 30% and 70% of Percoll (GE healthcare) were isolated by density-cut centrifugation as described38. These cells were cultured for 4 days and then activated by TNF-α (10 ng/ml, BioLegend) and IL-17 (25 ng/ml, BioLegend) in the presence and absence of SCFAs (C2 at 10 mM, C3 at 1 mM, C4 at 0.5 mM) for additional 3 days in DMEM medium (10% FBS). Adherent glial cells, largely composed of microglial cells, were co-cultured as APCs with naïve CD4+ T cells at 1:10 ratio for 5–6 days in the presence of Staphylococcal enterotoxin B (SEB, 5 μg/ml, List Labs) to stimulate naïve CD4+ T cells isolated from the spleen and lymph nodes of unimmunized C57BL/6 mice.

PubMed ID: 31222050

Distinct Spatiotemporal Distribution of Bacterial Toxin-Produced Cellular cAMP Differentially Inhibits Opsonophagocytic Signaling

Hasan, S;Rahman, WU;Sebo, P;Osicka, R;
Product: Anthrax Edema Factor (EF), Recombinant from B. anthracis
- as determined by Limulus Amebocyte Lysate assay (QCL-1000, Lonza, Walkersville, MD) and was stored in 50 mM Tris (pH 8.0), 8 M urea, and 2 mM CaCl 2 buffer at ‒20 °C. Recombinant EF and PA from Bacillus anthracis were purchased from List Biological Laboratories Cached
PubMed ID: 31226835

HSC70 and HSP90 chaperones perform complementary roles in translocation of the cholera toxin A1 subunit from the endoplasmic reticulum to the cytosol

Burress, H;Kellner, A;Guyette, J;Tatulian, SA;Teter, K;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
- Sigma- Aldrich (St. Louis, MO). DT and CT were purchased from List Biological Laboratories (Campbell, CA). His-tagged CTA1 and 13C- labeled CTA1 were purified in the lab as previously described (49,62). HSC70 was purchased
PubMed ID: 31221799

Pyruvate kinase M2 is requisite for Th1 and Th17 differentiation

Kono, M;Maeda, K;Stocton-Gavanescu, I;Pan, W;Umeda, M;Katsuyama, E;Burbano, C;Orite, SYK;Vukelic, M;Tsokos, MG;Yoshida, N;Tsokos, GC;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Five million cells were suspended in 150 μl of PBS (pH 7.4) and injected intravenously into each Rag1-deficient mouse. Pertussis toxin (300 ng per mouse; List Biological Laboratories) was intraperitoneally injected later on the day of transfer and 2 days later. Mice were monitored and weighed as previously described (11).
PubMed ID: 31217348