For PT-specific IgG ELISA, plates were coated with 0.5 µg/mL purified PT (List Biologicals Campbell, California) in PBS and the assay performed as previously described . ...
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).
Infection and stimulation of monocytes:
... Cells were stimulated with 100 ng/ml ultrapure E. coli LPS (List Biological Laboratories, Campbell, CA) and 5 mM ATP (Sigma-Aldrich, St. Louis, MO) for the last 30 min of culture, as indicated. “Mock” treatment was the addition of the equivalent volume of media (without parasites or LPS) to cells. At the indicated time point, monocytes were pelleted by centrifugation at 500 x g for 5 min. Collected cells were stained, fixed, or lysed accordingly, as described below.
Author did not specify which ULTRA PURE E. coli LPS was utilized; List Labs provides the following: